Human Calbindin(CALB1) ELISA kit

Instructions
Code CSB-EL004432HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name calbindin 1, 28kDa
Alternative Names avian-type ELISA Kit; CAB27 ELISA Kit; CALB 1 ELISA Kit; CALB ELISA Kit; CALB1 ELISA Kit; CALB1_HUMAN ELISA Kit; Calbindin 1 28kDa ELISA Kit; Calbindin ELISA Kit; Calbindin D28 ELISA Kit; D 28K ELISA Kit; D-28K ELISA Kit; D28K ELISA Kit; OTTHUMP00000166027 ELISA Kit; OTTHUMP00000225441 ELISA Kit; RTVL H protein ELISA Kit; Vitamin D dependent calcium binding protein ELISA Kit; Vitamin D dependent calcium binding protein avian type ELISA Kit; Vitamin D-dependent calcium-binding protein ELISA Kit
Abbreviation CALB1
Uniprot No. P05937
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human calbindin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-99  
1:2 Average % 92  
Range % 88-96  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 84  
Range % 80-88  
Recovery
The recovery of human calbindin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 102 95-110  
EDTA plasma (n=4) 98 93-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.178 2.302 2.240 2.039  
500 1.574 1.532 1.553 1.352  
250 1.054 1.076 1.065 0.864  
125 0.764 0.791 0.778 0.577  
62.5 0.478 0.452 0.465 0.264  
31.25 0.351 0.382 0.367 0.166  
15.6 0.266 0.258 0.262 0.061  
0 0.197 0.204 0.201    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Gene References into Functions
  1. CSF calbindin concentration is a sensitive biomarker of NPC1 disease PMID: 27307499
  2. We identified a significant association between the A allele of rs1805874 and the risk of Parkinson's disease (OR 1.257, 95% CI 1.036-1.524, p = 0.020). Our findings suggest that the A allele of rs1805874 is associated with risk of Parkinson's disease among the Han Chinese. Our results, combined with previous studies, suggest that rs1805874 is associated with Parkinson's disease in East Asians, but not Caucasians. PMID: 27611799
  3. Age-related loss of CB appears to coincide with tangle formation in the BFCN and is associated with the full range of tau pathology, including late appearing epitopes. PMID: 26417681
  4. Increased number of calbindin neurons and fiber labeling both in focal cortical dysplasia (FCD) and non-FCD specimens compared with postmortem controls may be associated with ongoing seizure activity. PMID: 26081613
  5. expressed by different neuron types, both inhibitory (GABAergic) and excitatory (glutamatergic), involved in both intrinsic and extrinsic circuits of the human cerebellar cortex PMID: 24719368
  6. The calbindin-D 28k was decreased in the putamen, the dorsal tier of the substantia nigra along the lower border of the red nucleus, and in the cerebellar cortex in multiple system atrophy. PMID: 23715974
  7. Significantly reduced hippocampal calbindin D28K levels are found in all patients with mitochondrial encephalomyopathy, lactic acidosis and stroke-like syndrome (MELAS). PMID: 22483853
  8. Data show calbindin (CB)- and tyrosine hydroxylase (TH)-cells were distributed in the three striatal territories, and the density of calretinin (CR) and parvalbumin (PV) interneurons were more abundant in the associative and sensorimotor striatum. PMID: 22272358
  9. Dynamics of Ca2+ ions within the solvation shell of calbindin D9k. PMID: 21364983
  10. upregulated in endometriosis tissues PMID: 20452033
  11. cultured syncytiotrophoblast cells express calbindin-D9k and calbindin-D28k genes, which are stimulated by calcitriol PMID: 20214988
  12. Our study shows there is no association between rs1805874 and risk for Parkinson's disease (PD) in four Caucasian populations. This suggests the effect of calbindin on PD risk displays population specificity. PMID: 19674066
  13. calbindin may play a role in development of Purkinje cells located in heterotopias and cerebellar dysgenesias PMID: 20054781
  14. S-nitrosation of Ca(2+)-loaded and Ca(2+)-free recombinant calbindin D(28K) from human brain. PMID: 11994015
  15. binds to myo-inositol-1(or 4)-monophosphatase (IMPase) and increases the phosphatase activity PMID: 12176979
  16. calbindin 1, 28kDa may play a role in Ca2+ transport or cell development in human trophoblast possibly trough Ca2+ buffering PMID: 12606474
  17. The biological function of calbindin D28k appears to be tied to the redox state of its five cysteine residues. PMID: 15641794
  18. Post-translational modifications of human calbindin. PMID: 15741335
  19. Our findings indicate that the expression of parvalbumin or calbindin-D(28k) by subpopulations of dysplastic neurons in cortical tubers is aberrant and denotes dysfunctional inhibitory circuits inept for excitoprotection. PMID: 17156698
  20. aggregates of mutant ataxin-l may recruit calbindin-D28k via tissue transglutaminase 2 mediated covalent crosslinking PMID: 17442486
  21. recombinant human calbindin-D28k was crystallized at 291 K using PEG 3350 as precipitant and a 2.4 A resolution X-ray data set was collected from a single flash-cooled crystal PMID: 18259068
  22. calbindin D(28k) binds Zn2+ to 3 rather strong sites with dissociation constants in low micromolar range. Zn2+-bound state is structurally distinct from Ca2+-bound state, with negative allosteric interaction between Zn- & Ca-binding events. PMID: 18359862
  23. CALB1 is associated with Parkinson's disease independently of alpha-synuclein. PMID: 18568448
  24. No significant differences in cerebellar Purkinje cells in levels of CALB in autism or in the normal cerebellum. PMID: 18587625

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Protein Families Calbindin family
Database Links

HGNC: 1434

OMIM: 114050

KEGG: hsa:793

STRING: 9606.ENSP00000265431

UniGene: Hs.65425

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