Human Carbonic Anhydrase 3(CA3) ELISA Kit

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Target Background

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Product Details

Description

This Human CA3 ELISA Kit was designed for the quantitative measurement of Human CA3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL.

Target Name

carbonic anhydrase III, muscle specific

Alternative Names

CA III ELISA Kit; CA-III ELISA Kit; Ca3 ELISA Kit; CAH3_HUMAN ELISA Kit; CAIII ELISA Kit; Car3 ELISA Kit; Carbonate dehydratase III ELISA Kit; Carbonic anhydrase 3 ELISA Kit; Carbonic anhydrase III ELISA Kit; Carbonic anhydrase III muscle specific ELISA Kit; EC 4.2.1.1 ELISA Kit; Epididymis secretory sperm binding protein Li 167mP ELISA Kit; HEL S 167mP ELISA Kit

Abbreviation

CA3

Uniprot No.

P07451

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8 ng/mL-500 ng/mL

Sensitivity

1.95 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	95
	Range %	90-105
1:4	Average %	103
	Range %	97-107
1:8	Average %	89
	Range %	85-95

Recovery

The recovery of human CA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.748	2.579	2.664	2.512
250	2.187	2.136	2.162	2.010
125	1.467	1.488	1.478	1.326
62.5	0.897	0.722	0.810	0.658
31.2	0.488	0.490	0.489	0.337
15.6	0.346	0.345	0.346	0.194
7.8	0.225	0.237	0.231	0.079
0	0.156	0.148	0.152

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Background

Function (From Uniprot)	Reversible hydration of carbon dioxide.
Gene References into Functions	CAIII and Hsp70 expressions were higher in LPRD patients that in non-LPRD patients, suggesting the possibility as one of biomomarker in LPRD diagnosis. PMID: 26847302 this study detected the formation and colocalization of 6-nitrotryptophan-containing proteins and CAIII in the skin of atopic dermatitis patients, not only in the lesional part but also in the nonlesional part, through histochemical analyses PMID: 24838180 Report that anti-CAIII antibodies may be useful for diagnosing microscopic polyangiitis. PMID: 23981757 The carbonic anhydrase CA3 isoform displayed an increased abundance during muscle aging, which was independently verified by immunoblotting of differently aged human skeletal muscle samples. PMID: 22797148 The generation of CA III and IV autoantibodies, antioxidant enzymes, and cytokines might influence each other. PMID: 23049597 Crystallization and preliminary X-ray analysis of human carbonic anhydrase III PMID: 11976500 Data show that inserting histidine residues into the active site cavity of carbonic anhydrase II or III results in rates of proton transfer to the zinc-bound hydroxide that are antagonistic or suppressive with respect to the corresponding single mutants. PMID: 12171926 Proton transfer within the active-site cavity of carbonic anhydrase III PMID: 12484342 myoglobin/carbonic anhydrase III ratio in the blood proved to be a more specific indicator for myocardial damage than myoglobin alone after myocardial infarction. PMID: 12745799 CAIII expression is upregulated in kidney cortex samples from the end-stage kidney of a patient with Dent's disease owing to the G506E mutation of CLCN5 PMID: 18322545 CAIII promotes transformation and invasion capability in hepatoma cells through FAK signaling pathway. PMID: 18444244 The level of CAIII is specifically insufficient in the skeletal muscle of myasthenia gravis patients. PMID: 19301202 Show More Hide All
Subcellular Location	Cytoplasm.
Protein Families	Alpha-carbonic anhydrase family
Tissue Specificity	Muscle specific.
Database Links	HGNC: 1374 OMIM: 114750 KEGG: hsa:761 STRING: 9606.ENSP00000285381 UniGene: Hs.82129

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Check Code：

Code	CSB-E15962h
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.