Human Carbonic Anhydrase 3(CA3) ELISA Kit

Instructions
Code CSB-E15962h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name carbonic anhydrase III, muscle specific
Alternative Names CA III ELISA Kit; CA-III ELISA Kit; Ca3 ELISA Kit; CAH3_HUMAN ELISA Kit; CAIII ELISA Kit; Car3 ELISA Kit; Carbonate dehydratase III ELISA Kit; Carbonic anhydrase 3 ELISA Kit; Carbonic anhydrase III ELISA Kit; Carbonic anhydrase III muscle specific ELISA Kit; EC 4.2.1.1 ELISA Kit; Epididymis secretory sperm binding protein Li 167mP ELISA Kit; HEL S 167mP ELISA Kit
Abbreviation CA3
Uniprot No. P07451
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-96  
1:2 Average % 95  
Range % 90-105  
1:4 Average % 103  
Range % 97-107  
1:8 Average % 89  
Range % 85-95  
Recovery
The recovery of human CA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 97 90-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
500 2.748 2.579 2.664 2.512  
250 2.187 2.136 2.162 2.010  
125 1.467 1.488 1.478 1.326  
62.5 0.897 0.722 0.810 0.658  
31.2 0.488 0.490 0.489 0.337  
15.6 0.346 0.345 0.346 0.194  
7.8 0.225 0.237 0.231 0.079  
0 0.156 0.148 0.152    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Reversible hydration of carbon dioxide.
Gene References into Functions
  1. CAIII and Hsp70 expressions were higher in LPRD patients that in non-LPRD patients, suggesting the possibility as one of biomomarker in LPRD diagnosis. PMID: 26847302
  2. this study detected the formation and colocalization of 6-nitrotryptophan-containing proteins and CAIII in the skin of atopic dermatitis patients, not only in the lesional part but also in the nonlesional part, through histochemical analyses PMID: 24838180
  3. Report that anti-CAIII antibodies may be useful for diagnosing microscopic polyangiitis. PMID: 23981757
  4. The carbonic anhydrase CA3 isoform displayed an increased abundance during muscle aging, which was independently verified by immunoblotting of differently aged human skeletal muscle samples. PMID: 22797148
  5. The generation of CA III and IV autoantibodies, antioxidant enzymes, and cytokines might influence each other. PMID: 23049597
  6. Crystallization and preliminary X-ray analysis of human carbonic anhydrase III PMID: 11976500
  7. Data show that inserting histidine residues into the active site cavity of carbonic anhydrase II or III results in rates of proton transfer to the zinc-bound hydroxide that are antagonistic or suppressive with respect to the corresponding single mutants. PMID: 12171926
  8. Proton transfer within the active-site cavity of carbonic anhydrase III PMID: 12484342
  9. myoglobin/carbonic anhydrase III ratio in the blood proved to be a more specific indicator for myocardial damage than myoglobin alone after myocardial infarction. PMID: 12745799
  10. CAIII expression is upregulated in kidney cortex samples from the end-stage kidney of a patient with Dent's disease owing to the G506E mutation of CLCN5 PMID: 18322545
  11. CAIII promotes transformation and invasion capability in hepatoma cells through FAK signaling pathway. PMID: 18444244
  12. The level of CAIII is specifically insufficient in the skeletal muscle of myasthenia gravis patients. PMID: 19301202

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Subcellular Location Cytoplasm
Protein Families Alpha-carbonic anhydrase family
Tissue Specificity Muscle specific.
Database Links

HGNC: 1374

OMIM: 114750

KEGG: hsa:761

STRING: 9606.ENSP00000285381

UniGene: Hs.82129

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