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Human Carboxypeptidase E(CPE) ELISA kit

Protocol
Code CSB-EL005886HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
carboxypeptidase E
Alternative Names
Carboxypeptidase E ELISA Kit; Carboxypeptidase H ELISA Kit; Carboxypeptidase H precursor ELISA Kit; CarboxypeptidaseE ELISA Kit; CarboxypeptidaseH ELISA Kit; CBPE_HUMAN ELISA Kit; Cobalt stimulated chromaffin granule carboxypeptidase ELISA Kit; CPE ELISA Kit; Cph 1 ELISA Kit; CPH ELISA Kit; Enkephalin convertase ELISA Kit; Entephalin convertase ELISA Kit; Insulin granule associated carboxypeptidase ELISA Kit; Prohormone processing carboxypeptidase ELISA Kit; Prohormone-processing carboxypeptidase ELISA Kit
Abbreviation
CPE
Uniprot No.
P16870
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
39.06 pg/mL-2500 pg/mL
Sensitivity
9.76 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 105
Range % 94-109
1:2 Average % 107
Range % 98-111
1:4 Average % 92
Range % 84-95
1:8 Average % 98
Range % 90-102
Recovery
The recovery of human CPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 84-94
EDTA plasma (n=4) 101 95-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2500 2.410 2.333 2.372 2.246
1250 1.712 1.679 1.696 1.570
625 1.029 0.968 0.999 0.873
312.5 0.634 0.655 0.645 0.519
156.25 0.410 0.401 0.406 0.280
78.12 0.280 0.284 0.282 0.156
39.06 0.218 0.225 0.222 0.096
0 0.125 0.127 0.126 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CPE ELISA Kit was designed for the quantitative measurement of Human CPE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39.06 pg/mL-2500 pg/mL and the sensitivity is 9.76 pg/mL.

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Target Background

Function
(From Uniprot)
Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.
Gene References into Functions
  1. Our results implicate a novel role for Carboxypeptidase E that mainly affects the expression of motility-associated genes via several signal pathways PMID: 28656234
  2. This study has uncovered a human CPE/NF-alpha1 gene mutation that could lead to comorbidity of dementia and depression, emphasizing the importance of this gene in cognitive function. PMID: 27922637
  3. CPE through its N'-terminal sequence, forms aggregates with Wnt3a and possible endoplasmic reticulum (ER) stress leading to its loss of function. PMID: 27375026
  4. we have identified a novel SNP in the CPE gene which results in the loss of its neuroprotective function in cells and may confer neurological disorders in humans PMID: 28114332
  5. High-level CPE [ carboxypeptidase E] expression was associated with a poor prognosis in early-stage cervical cancer. CPE may serve as a biomarker for predicting PLNM [ pelvic lymph node metastasis ] and survival in these patients. PMID: 26695643
  6. Low carboxypeptidase E expression is associated with recurrence in early-stage hepatocellular carcinoma. PMID: 26803519
  7. Down-expression of liver carboxypeptidase E may reduce the secretion of serum cholecystokinin and contribute to the formation of cholesterol gallstone. PMID: 26228366
  8. Downregulation of CPE regulates cell proliferation and chemosensitivity in pancreatic cancer. PMID: 25374060
  9. Disruption of insulin receptor (IR) expression in beta cells has a direct impact on the expression of the convertase enzyme carboxypeptidase E (CPE) by inhibition of the eukaryotic translation initiation factor Eif4g1. PMID: 24843127
  10. Upregulation of CPE promotes cell proliferation and tumorigenicity in colorectal cancer. PMID: 24006921
  11. Data suggest that splice variant of carboxypeptidase E (CPE-DeltaN) that CPE-DeltaN expression might be a potential prognostic marker for colorectal cancer patients. PMID: 23852859
  12. CPE is essential in the process and targeting of neuropeptides and neurotrophins, its participation in the pathological progression of Alzheimer's disease may be suggested PMID: 22998035
  13. CPE forms a complex, probably through sequences located at its N-terminal domain, with Wnt3a and the extracellular cysteine rich domain of Fz1. PMID: 22824791
  14. Neither high glucose nor insulin (with low glucose) regulates beta-cell CPE (but either up-regulates CPD). PMID: 21628999
  15. an N-terminal truncated carboxypeptidase E splice isoform induces tumor growth and is a biomarker for predicting future metastasis in human cancers PMID: 21285511
  16. CPE may play a role in promoting tumor growth and invasion. PMID: 21061162
  17. Carboxypeptidase E is differently expressed in subcutaneous and visceral fat of obese subjects PMID: 12530526
  18. protein binding with Con A in seminal plasma PMID: 14690244
  19. cDNA microarray analysis led to the identification of 2 novel biomarkers that should facilitate molecular diagnosis and further study of pulmonary neuroendocrine tumors. PMID: 15492986
  20. A possible role for mutations in CPE in the development of coronary heart disease. PMID: 17957445
  21. the severity of the coronary atherosclerosis estimated by Gensini score was significantly influenced by the presence of the A2925G mutant and G2855A mutant of the CPE gene PMID: 18080843
  22. polymorphism in the CPE exon5 gene may contribute to the angiographical characteristics of coronary atherosclerosis in the Chinese population PMID: 18501121
  23. Carboxypeptidase E degradation contributes to palmitate-induced beta-cell ER stress and apoptosis; CPE is a major link between hyperlipidemia and beta-cell death pathways in diabetes. PMID: 18550819
  24. CPH-Abs may allow discrimination of a more latent subset of adult-onset autoimmune diabetes (LADA). PMID: 19120309
  25. Carboxypeptidase E may be a key molecule to regulate Caspr2 (carboxypeptidase E) trafficking to the cell membrane. PMID: 19166515
  26. missense polymorphism encoding altered activity PMID: 11462236

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Subcellular Location
[Isoform 1]: Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted.
Protein Families
Peptidase M14 family
Database Links

HGNC: 2303

OMIM: 114855

KEGG: hsa:1363

STRING: 9606.ENSP00000386104

UniGene: Hs.75360

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