Human Caspase 8(Casp-8) ELISA Kit

Code CSB-E13636h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q14790
Lead Time 3-5 working days
Abbreviation CASP8
Protein Biological Process 1 Apoptosis/Autophagy
Target Name caspase 8, apoptosis-related cysteine peptidase
Alias ALPS2B, CAP4, Casp-8, FLICE, FLJ17672, MACH, MCH5, MGC78473, FADD-homologous ICE/CED-3-like protease|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|OTTHUMP00000163720|caspase 8|caspase 8, apopto
Species Homo sapiens (Human)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
HGNC 1876
RGD 620847
MGI 1336166
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CASP-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 80-96  
1:2 Average % 90  
Range % 84-102  
1:4 Average % 95  
Range % 92-100  
1:8 Average % 93  
Range % 83-99  
Recovery
The recovery of human CASP-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 87-103  
EDTA plasma (n=4) 97 90-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.226 2.238 2.232 2.136  
1000 1.513 1.546 1.530 1.434  
500 0.917 0.932 0.925 0.829  
250 0.588 0.579 0.584 0.488  
125 0.406 0.394 0.400 0.304  
62.5 0.251 0.237 0.244 0.148  
31.25 0.139 0.133 0.136 0.040  
0 0.095 0.097 0.096    
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Function Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Involvement in disease Caspase-8 deficiency (CASP8D)
Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
Database Links

HGNC: 1509

OMIM: 211980

KEGG: hsa:841

STRING: 9606.ENSP00000351273

UniGene: Hs.599762

Pathway p53 signaling pathway
TNF signaling pathway
Apoptosis
Necroptosis
IL-17 signaling pathway
NOD-like receptor signaling pathway
Toll-like receptor signaling pathway

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