Human Cholesterol 7α-Hydroxylase (CYP7A)ELISA Kit

Code CSB-E14344h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P22680
Lead Time 3-5 working days
Protein Biological Process 2 Lipogenesis and lipometabolism
Abbreviation CYP7A1
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name cytochrome P450, family 7, subfamily A, polypeptide 1
Alias CP7A, CYP7, MGC126826, MGC138389, cholesterol 7 alpha-monooxygenase|cholesterol 7-alpha-hydroxylase|cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1
Species Homo sapiens (Human)
Protein Biological Process 3 Cholesterol metabolism
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body.
HGNC 2651
RGD 2482
MGI 106091
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CYP7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %94
Range %90-100
1:4Average %92
Range %85-97
1:8Average %92
Range %87-98
Recovery
The recovery of human CYP7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-98
EDTA plasma (n=4)9185-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.606 2.520 2.563 2.420
6001.912 1.769 1.841 1.698
3001.070 1.004 1.037 0.894
1500.686 0.639 0.663 0.520
750.408 0.394 0.401 0.258
37.50.314 0.309 0.312 0.169
18.750.216 0.199 0.208 0.065
00.149 0.137 0.143  
References
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Function Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
Subcellular Location Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane, Peripheral membrane protein
Protein Families Cytochrome P450 family
Tissue Specificity Detected in liver.
Database Links

HGNC: 2651

OMIM: 118455

KEGG: hsa:1581

STRING: 9606.ENSP00000301645

UniGene: Hs.1644

Pathway PPAR signaling pathway
Cholesterol metabolism

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