Human Cholesterol 7α-Hydroxylase (CYP7A)ELISA Kit

Instructions
Code CSB-E14344h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name cytochrome P450, family 7, subfamily A, polypeptide 1
Alternative Names Cholesterol 7 alpha hydroxylase ELISA Kit; Cholesterol 7 alpha monooxygenase ELISA Kit; Cholesterol 7-alpha-hydroxylase ELISA Kit; Cholesterol 7-alpha-monooxygenase ELISA Kit; CP7A ELISA Kit; CP7A1_HUMAN ELISA Kit; CYP 7 ELISA Kit; CYP7 ELISA Kit; CYP7A1 ELISA Kit; CYPVII ELISA Kit; Cytochrome P450 7A1 ELISA Kit; Cytochrome P450; family 7; subfamily A; polypeptide 1 ELISA Kit; Cytochrome P450; subfamily VIIA (cholesterol 7 alpha monooxygenase); ELISA Kit; Cytochrome P450; subfamily VIIA (cholesterol 7 alpha-monooxygenase); polypeptide 1 ELISA Kit; MGC126826 ELISA Kit; MGC138389 ELISA Kit
Abbreviation CYP7A1
Uniprot No. P22680
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CYP7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %94
Range %90-100
1:4Average %92
Range %85-97
1:8Average %92
Range %87-98
Recovery
The recovery of human CYP7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-98
EDTA plasma (n=4)9185-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.606 2.520 2.563 2.420
6001.912 1.769 1.841 1.698
3001.070 1.004 1.037 0.894
1500.686 0.639 0.663 0.520
750.408 0.394 0.401 0.258
37.50.314 0.309 0.312 0.169
18.750.216 0.199 0.208 0.065
00.149 0.137 0.143  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
Gene References into Functions
  1. miR-17 is a novel regulator of CYP7A1 signaling in hepatic lipid metabolism, by which miR-17 may mediate hepatosteatosis. PMID: 29721023
  2. Rev-erbalpha regulates Cyp7a1 and cholesterol metabolism through its repression of the Lrh-1 receptor. PMID: 29237721
  3. The analysis of the relationship of polymorphic variants CYP7A1 to lipid metabolic disturbances makes it possible to consider the AA homozygous genotype of variant mutation CYP7A1 rs 38088607 as protective against Dyslipidemia. PMID: 29171472
  4. Genetic polymorphisms of CYP7A1 may be associated with susceptibility to anti-tuberculosis drug-induced hepatotoxicity in the Chinese population PMID: 27155186
  5. The pronounced TC reduction in G allele carriers of rs3808607 observed in the previous study may be due to enhanced bile acid synthesis in response to high-viscosity beta-glucan consumption in those individuals. PMID: 29115200
  6. Current studies suggest that increased Cyp7a1 expression and bile acid synthesis ameliorate hepatic inflammation through activation of FXR PMID: 27534992
  7. This study provides evidence that individuals carrying the G allele of CYP7A1 single nucleotide polymorphism are more responsive to high molecular weight beta-glucan's effect of lowering circulating cholesterol concentrations than individuals homozygous for the T allele. PMID: 26936139
  8. Genetic variations in ABCG5, CYP7A1, and DHCR7 may contribute to differing responses of serum cholesterol to dairy intake among healthy adults. PMID: 27052530
  9. The promoter polymorphism of the CYP7A1 gene has a pronounced impact on diurnal variation in CYP7A1 activity. PMID: 27106353
  10. The finding that overexpression of HIF-1alpha increased the activity of the CYP7A1 promoter suggested that hypoxia decreased the expression of CYP7A1 in a HIF-1-independent manner. PMID: 26521940
  11. Macrophage cholesterol efflux in patients with type II diabetes mellitus was significantly reduced, and that this reduction was associated with the down-regulation of CYP7A1 expression. PMID: 26345803
  12. CYP7A1 and APOE isoform are associated with the extent of reduction in circulating LDL cholesterol in response to plant sterols consumption. PMID: 26333513
  13. The CYP7A1 rs7833904 polymorphism may modify the risk of CAD. PMID: 25944972
  14. The CYP7A1 crystallographic models identify residues involved in cholest-4-en-3-one binding. PMID: 24927729
  15. genetic polymorphism is associated with the total cholesterol level and the lipid-lowering efficacy of statin treatment. [meta-analysis] PMID: 25499945
  16. we identified for the first time a significant association of the A-204C polymorphism of the CYP7A1 gene and development of tuberculosis in a Moroccan population PMID: 25360185
  17. The CYP7A1 -204A>C polymorphism is significantly associated with serum lipid levels in Asian population, but not gallbladder stone disease. [Meta-analysis] PMID: 25103562
  18. Cyp7a1 is a direct Thyroid hormone receptor (TR) target gene that responds to physiologic TR levels through a set of distinct response elements in its promoter. PMID: 24582860
  19. Activation of the VDR represses hepatic SHP to increase levels of CYP7A1 and reduce cholesterol. PMID: 24365583
  20. Two known single nucleotide polymorphisms were identified in the 5'-UTR of CYP7A1 and both were associated with neuromyelitis optica but not with Multiple sclerosis. PMID: 23740208
  21. Genetic variants of CYP7A1 and its transcriptional activators (HNF4A and PPARGC1A) may activate bile acid synthesis, resulting in the accumulation of bile acids in hepatocytes leading primary biliary cirrhosis progression. PMID: 23354620
  22. Prox1 interacts with LSD1 to recruit the repressive LSD1/NuRD complex to CYP7A1 promoter and co-represses transcription through epigenetic mechanisms. PMID: 23626788
  23. A variant of COUP-TFII downregulates the function of COUP-TFII by inhibiting its binding to DNA, decreasing Cyp7a1 expression. PMID: 23458092
  24. HNF4alpha and LRH-1 promote active transcription histone marks on the Cyp7a1 promoter that are reversed by FGF19 in a SHP-dependent manner PMID: 23038264
  25. Liver X receptor alpha is required for induction of trascription of CYP7A1 in response to resveratrol. PMID: 22607622
  26. these data suggest that FGF7 is a novel regulator of CYP7A1 expression in hepatocytes and may prevent hepatocytes from accumulating toxic bile acids during liver injury and fibrosis. PMID: 22713451
  27. gender, but not SLCO1B1 or CYP7A1 polymorphism, has a major effect on the fasting plasma concentrations of individual bile acids PMID: 21902813
  28. CYP7A1 polymorphisms are associated with colorectal adenoma. PMID: 22058145
  29. The main enzyme regulating bile acids biosynthesis is CYP7A1 (7alpha-cholesterol hydroxylase). [review] PMID: 22235657
  30. 7-dehydrocholesterol (the immediate precursor of cholesterol) is oxidized by P450 7A1 to 7-ketocholesterol. PMID: 21813643
  31. The frequencies of rs3808607 alleles in the CYP7A1 gene differed significantly between obese hypertensive and normotensive men. PMID: 21346769
  32. Results suggest that promoter -204A > C variant is associated with enhanced CYP7A1 activity. PMID: 20884100
  33. Cytochrome P450 7A1 cholesterol 7alpha-hydroxylation: individual reaction steps in the catalytic cycle and rate-limiting ferric iron reduction. PMID: 21147774
  34. The mRNA expression levels of sodium taurocholate cotransporting polypeptide, bile salt export pump, and hepatic cholesterol 7alpha-hydroxylase were significantly higher in the primary biliary cirrhosis patients than in the controls. PMID: 20857261
  35. These results demonstrate that no association exists between apoB-100, apoE, and CYP7A1 polymorphisms and cholelithiasis in a Mexican population. PMID: 20872969
  36. study found glucose positively regulated hepatocyte CYP7A1 gene & bile acid synthesis; this glucose-mediated induction of CYP7A1 transcription may be mediated by both AMPK-dependent pathway & epigenetic regulation of CYP7A1 chromatin structure PMID: 19965590
  37. miR-122a and miR-422a may destabilize CYP7A1 mRNA to inhibit CYP7A1 expression. PMID: 20351063
  38. insulin is involved in the regulation of diurnal variation of CYP7A1 activity in humans PMID: 19537927
  39. Statistical analyses revealed that a common promoter SNP in CYP7A1 has a protective/gene dose-dependent effect on the risk of NMO. PMID: 19850125
  40. mechanism for gallbladder cancer susceptibility by CYP7A1 haplotype appears to be independent of gallstone pathway and is believed to involve genotoxicity resulting from subnormal bile acid production PMID: 20005541
  41. The study provides evidence that -278A>C polymorphism in the CYP7A1 gene can modify triglyceride concentrations in response to a reduced fat diet in a dyslipidemic male population. PMID: 19448895
  42. CYP7A1 regulates the pathway through which cholesterol is converted into bile acids. PMID: 11907135
  43. regulation of CYP7A1 and CYP27A1 in human liver PMID: 12011083
  44. Human CYP7A1 deficiency: progress and enigmas. Review. PMID: 12093884
  45. New metabolic disorder presenting with hyperlipidemia caused by a homozygous deletion mutation in CYP7A1. PMID: 12093894
  46. data suggest that the lack of an LXR element in the region from -56 to -49 of the human CYP7A1 promoter may account for some of the differences in response to diets between humans and rodents PMID: 12202481
  47. characterization of the coordinated regulation of cholesterol metabolism in human liver; regulation of its mRNA in liver PMID: 12213890
  48. Data reveal a fundamental difference in the regulation of CYP7A1 in rodent and human hepatocytes. PMID: 12554795
  49. demonstrated that FGF-19, acting as an FXR-induced signaling molecule, represses expression of the CYP7A1 gene; this signaling cascade defines a novel mechanism for feedback repression of bile acid biosynthesis PMID: 12815072
  50. bile acids suppress transcription of the gene (CYP7A1) encoding cholesterol 7alpha-hydroxylase, the rate-limiting enzyme in bile acid biosynthesis PMID: 12865425
  51. CYP7A1 is activated by PGC-1alpha PMID: 14522988
  52. data indicate that thyroid hormone can repress the human cholesterol 7 alpha-hydroxylase(CYP7A1) gene in transgenic mice, but this effect is dependent on gender PMID: 14592954
  53. dietary cholesterol regulates bile acid pool size, fecal bile acid excretion, and plasma cholesterol independently of Cyp7a1 activity PMID: 14762172
  54. The liver receptor homolog-1 has emerged as an essential regulator for the expression of CYP7A1. PMID: 15205472
  55. CYP7A1 polymorphism has a small but significant effect on the increase in plasma HDL cholesterol and plasma total cholesterol after an increased intake of dietary cholesterol and cafestol, respectively. PMID: 15333704
  56. Present study indicates that a tight fit of cholesterol in the CYP7A1 active site is in part responsible for the high efficiency of cholesterol turnover by CYP7A1 in the liver. PMID: 15736936
  57. This study has found that several kinase activators rapidly reduce the amount of bile acid produced by the human hepatoma cell line, HepG2, and that gpCYP7A1 from HepG2 cell extracts eluted in the phosphoprotein fraction of FeIII columns. PMID: 15752749
  58. regulation by HNF-4alpha PMID: 15796896
  59. common polymorphisms of the CYP7A1 gene do not contribute to variation in cholesterol 7alpha-hydroxylase activity, rates of bile acid synthesis and plasma LDL cholesterol concentration in humans PMID: 16115473
  60. The findings add to evidence for the role of bile acids in colorectal carcinogenesis. The CC genotype of the CYP7A1 A-203C polymorphism probably renders lower activity of the enzyme synthesizing bile acids. PMID: 16630139
  61. A constant genetic structure in CYP7A1 gene and its surrounding sequences was found that may lead to a better design for association studies of genetic variations in CYP7A1 gene with cholesterol and bile acid metabolism. PMID: 16709249
  62. T(3) dose-dependently decreased total bile acid formation in parallel with decreased expression of CYP7A1 and CYP8B1 PMID: 16937432
  63. TGFbeta1 represses CYP7A1 gene transcription in human hepatocytes by a mechanism involving Smad3-dependent inhibition of HNF4alpha and histone deacetylase remodeling of CYP7A1 chromatin. PMID: 17920062
  64. the association of A-204C variation of CYP7A1 gene promoter polymorphism in gallbladder cancer patients, gallstone patients and healthy subjects PMID: 18178499
  65. Liver receptor homolog 1 (LRH-1) is a key transcriptional factor required for the hepatic expression of CYP7A1. PMID: 18270374
  66. CYP7A1 promoter polymorphism is not a valuable marker of gallstone disease susceptibility in a Polish population. PMID: 18307386
  67. PGC-1alpha is an important co-activator for LRH-1 and that SHP targets the interaction between LRH-1 and PGC-1alpha to inhibit CYP7A1 expression. PMID: 18385139
  68. The cholesterol 7 alpha-hydroxylase A-204C polymorphism was positively associated with the presence of atherosclerotic plaque and carotid and femoral intima-media thickness in health postmenopausal women PMID: 18665040
  69. the effect of a common -203A>C polymorphism in the CYP7A1 promoter region on the activity of CYP7A1 PMID: 18728290
  70. In the absence of changes in short heterodimer partner mRNA, CYP7A1 mRNA was strongly reduced in the liver of cholestatic patients in comparison with drained and control patients. PMID: 19185005
  71. Impaired regulation of FoxO1 may cause down-regulation of CYP7A1 gene expression and contribute to dyslipidemia in insulin resistance. PMID: 19463968
  72. Data show that 12 SNPs from CETP, APOAI, ABCB1, CYP7A1, and HMGCR genes to be associated with baseline LDL-C and high-density lipoprotein cholesterol levels and increased risk of CAD. PMID: 19558216
  73. common variant A203C of the CYP7A1 gene markedly affects cholesterol 7-alpha-hydroxylase activity in patients with severe bile salt malabsorption PMID: 18728290

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Subcellular Location Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane, Peripheral membrane protein
Protein Families Cytochrome P450 family
Tissue Specificity Detected in liver.
Database Links

HGNC: 2651

OMIM: 118455

KEGG: hsa:1581

STRING: 9606.ENSP00000301645

UniGene: Hs.1644

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