CYP7A1 Antibody

Datasheet
Code CSB-PA006460LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. P22680
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate
    All lanes: CYP7A1 antibody at 4.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • IHC image of CSB-PA006460LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human Cholesterol 7-alpha-monooxygenase protein (303-423AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500
Relevance Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Cholesterol 7-alpha-monooxygenase (EC 1.14.14.23) (CYPVII) (Cholesterol 7-alpha-hydroxylase) (Cytochrome P450 7A1), CYP7A1, CYP7
Immunogen Species Human
Research Area Cardiovascular
Target Names CYP7A1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
Subcellular Location Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane, Peripheral membrane protein
Protein Families Cytochrome P450 family
Tissue Specificity Detected in liver.
Database Links

HGNC: 2651

OMIM: 118455

KEGG: hsa:1581

STRING: 9606.ENSP00000301645

UniGene: Hs.1644

Pathway PPAR signaling pathway
Cholesterol metabolism

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