Human Chordin-like protein 1(CHRDL1) ELISA kit

Code CSB-EL005378HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
chordin-like 1
Alternative Names
CHL ELISA Kit; chordin like 1 ELISA Kit; Chordin-like protein 1 ELISA Kit; CHRDL1 ELISA Kit; CRDL1_HUMAN ELISA Kit; dA141H5.1 ELISA Kit; neuralin 1 ELISA Kit; Neuralin-1 ELISA Kit; Neurogenesin-1 ELISA Kit; NRLN1 ELISA Kit; Ventroptin ELISA Kit; VOPT ELISA Kit
Abbreviation
CHRDL1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %84-93
1:2Average %104
Range %98-107
1:4Average %96
Range %88-99
1:8Average %96
Range %91-99
Recovery
The recovery of human CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9084-94
EDTA plasma (n=4)9994-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.551 2.487 2.519 2.354
52.164 2.112 2.138 1.973
2.51.651 1.688 1.670 1.505
1.251.043 1.032 1.038 0.873
0.6250.660 0.645 0.653 0.488
0.3120.474 0.467 0.471 0.306
0.1560.268 0.272 0.270 0.105
00.163 0.167 0.165
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CHRDL1 ELISA Kit was designed for the quantitative measurement of Human CHRDL1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

Function
(From Uniprot)
Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development.
Gene References into Functions
  1. Finally, in vivo experiments confirmed that CHRDL1 acted as a tumor suppressor gene in suppressing tumor growth and metastasis PMID: 28423564
  2. Results show that Chrdl1 expression is upregulated in numerous breast cancer cells following TGF-beta stimulation and demonstrated that Chrdl1 acts as an inhibitor of BMP4-induced migration and invasion. PMID: 26976638
  3. These studies have identified and validated new factors enriched in juvenile chondrocytes as compared to adult chondrocytes including secreted extracellular matrix factors chordin-like 1 (CHRDL1) and microfibrillar-associated protein 4 (MFAP4). PMID: 26955889
  4. We provide the initial confirmation that X-linked megalocornea is associated with mutations in the CHRDL1 gene. PMID: 24073597
  5. CHRDL1 plays a key role in cornea homeostasis as evidenced by disease causing mutations in X-linked megalocornea. PMID: 25712132
  6. The detection of mutations in the CHRDL1 gene is useful for differential diagnosis with different forms of megalocornea. PMID: 26020825
  7. Novel CHRDL1 mutations in ten families with X-linked megalocornea, are reported. PMID: 25093588
  8. CHRDL1 is expressed in the developing human cornea and anterior segment in addition to the retina. PMID: 22284829
  9. Hypoxia-induced upregulation of CHL-1 alters the homeostatic balance between BMP-4 and VEGF to synergize with VEGF in driving retinal angiogenesis. PMID: 18587495
  10. reports the cloning of chick ventroptin and its importance in topographic retinotectal projection PMID: 11441185

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Involvement in disease
Megalocornea 1, X-linked (MGC1)
Subcellular Location
Secreted.
Tissue Specificity
Expressed in the developing cornea and in the eye anterior segment in addition to the retina. Differentially expressed in the fetal brain. There is high expression in cerebellum and neocortex. Expressed in retinal pericytes.
Database Links

HGNC: 29861

OMIM: 300350

KEGG: hsa:91851

STRING: 9606.ENSP00000361112

UniGene: Hs.496587

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