CHRDL1 Antibody

Datasheet

Code	CSB-PA866283LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate All lanes: CHRDL1 antibody at 5.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 44, 53 kDa Observed band size: 52 kDa IHC image of CSB-PA866283LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CHRDL1 Polyclonal antibody

Uniprot No.

Q9BU40

Target Names

CHRDL1

Alternative Names

CHL antibody; chordin like 1 antibody; Chordin-like protein 1 antibody; CHRDL1 antibody; CRDL1_HUMAN antibody; dA141H5.1 antibody; neuralin 1 antibody; Neuralin-1 antibody; Neurogenesin-1 antibody; NRLN1 antibody; Ventroptin antibody; VOPT antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Chordin-like protein 1 protein (92-237AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CHRDL1 Antibody (Product code: CSB-PA866283LA01HU) is Non-conjugated. For CHRDL1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA866283LB01HU	CHRDL1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA866283LC01HU	CHRDL1 Antibody, FITC conjugated
Biotin	CSB-PA866283LD01HU	CHRDL1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development.

Gene References into Functions

Finally, in vivo experiments confirmed that CHRDL1 acted as a tumor suppressor gene in suppressing tumor growth and metastasis PMID: 28423564
Results show that Chrdl1 expression is upregulated in numerous breast cancer cells following TGF-beta stimulation and demonstrated that Chrdl1 acts as an inhibitor of BMP4-induced migration and invasion. PMID: 26976638
These studies have identified and validated new factors enriched in juvenile chondrocytes as compared to adult chondrocytes including secreted extracellular matrix factors chordin-like 1 (CHRDL1) and microfibrillar-associated protein 4 (MFAP4). PMID: 26955889
We provide the initial confirmation that X-linked megalocornea is associated with mutations in the CHRDL1 gene. PMID: 24073597
CHRDL1 plays a key role in cornea homeostasis as evidenced by disease causing mutations in X-linked megalocornea. PMID: 25712132
The detection of mutations in the CHRDL1 gene is useful for differential diagnosis with different forms of megalocornea. PMID: 26020825
Novel CHRDL1 mutations in ten families with X-linked megalocornea, are reported. PMID: 25093588
CHRDL1 is expressed in the developing human cornea and anterior segment in addition to the retina. PMID: 22284829
Hypoxia-induced upregulation of CHL-1 alters the homeostatic balance between BMP-4 and VEGF to synergize with VEGF in driving retinal angiogenesis. PMID: 18587495
reports the cloning of chick ventroptin and its importance in topographic retinotectal projection PMID: 11441185

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Involvement in disease

Megalocornea 1, X-linked (MGC1)

Subcellular Location

Secreted.

Tissue Specificity

Expressed in the developing cornea and in the eye anterior segment in addition to the retina. Differentially expressed in the fetal brain. There is high expression in cerebellum and neocortex. Expressed in retinal pericytes.

Database Links

HGNC: 29861

OMIM: 300350

KEGG: hsa:91851

STRING: 9606.ENSP00000361112

UniGene: Hs.496587

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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