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Human Cytokine-like protein 1(CYTL1) ELISA kit

Instructions
Code CSB-EL006472HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cytokine-like 1
Alternative Names C17 ELISA Kit; C4orf4 ELISA Kit; CYTL1 ELISA Kit; CYTL1_HUMAN ELISA Kit; Cytokine like 1 ELISA Kit; Cytokine like protein C17 ELISA Kit; Cytokine-like protein 1 ELISA Kit; Protein C17 ELISA Kit
Abbreviation CYTL1
Uniprot No. Q9NRR1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CYTL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 87
Range % 82-91
1:2 Average % 94
Range % 89-98
1:4 Average % 94
Range % 90-97
1:8 Average % 92
Range % 88-97
Recovery
The recovery of human CYTL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 91-99
EDTA plasma (n=4) 103 98-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.008 2.102 2.055 1.877
5 1.735 1.797 1.766 1.588
2.5 1.408 1.369 1.389 1.211
1.25 1.121 1.068 1.095 0.917
0.625 0.737 0.728 0.733 0.555
0.312 0.488 0.468 0.478 0.300
0.156 0.336 0.331 0.334 0.156
0 0.178 0.178 0.178 　
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Citations

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CYTL1 Proteins

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Target Background

Gene References into Functions
  1. Circular dichroism analysis showed that, like chemokines, CYTL1has a higher content of beta-sheet than alpha-helix secondary structure; recombinant CYTL1 promoted calcium flux in chondrocytes; unlike chemokines, CYTL1 had limited affinity to proteoglycans; together, these properties further support cytokine-like properties for CYTL1 with some overlap with the chemokines PMID: 28478073
  2. this study shows that CYTL1 possesses chemotactic activity and demonstrates that its functional receptor is CCR2B PMID: 27084102
  3. when the native signal peptide of CYTL1 was replaced by IgGkappaSP, the expression and secretion was increased by 4 fold. PMID: 26922322
  4. role in regulation embryo implantation PMID: 26800213
  5. Cytl1 is highly expressed in the endometrium during the embryo implantation and its expression is regulated by ovarian hormones. Cytl1 enhances endometrial proliferation, induces the secretion of endometrial LIF and HB-EGF, and even enhances endometrial cell adhesion to trophoblastic cells. These indicate that Cytl1 is a potential molecular mediator of embryo is a potential molecular mediator in embryo implantation. PMID: 26800213
  6. our results showed the first evidence of CYTL1 expression in SH-SY5Y neuroblastoma cells and human NB tissues, revealed a possible link between CYTL1 and NB development PMID: 22797702
  7. evidence suggests that ANKRD7 and CYTL1 genes may play an important role in the variance in alcohol drinking risk PMID: 22613542
  8. C17orf62-L could induce cell death accompanied with rising of cleaved PARP. PMID: 21503106
  9. The DNA methylation pattern of the CYTL1 promoter region was significantly different between early and advanced stages of squamous cell carcinoma. PMID: 22011669
  10. C17 as a cytokine that s contributes to immune homeostasis systemically or in a tissue-specific manner in the joint PMID: 21799806
  11. We report a structural-functional characterization of cytokine-like protein 1 (Cytl1) by a combination of different computational structure-based techniques. PMID: 21322034

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Subcellular Location Secreted
Tissue Specificity Specifically expressed in CD34+ hematopoietic cells.
Database Links

HGNC: 24435

OMIM: 607930

KEGG: hsa:54360

STRING: 9606.ENSP00000303550

UniGene: Hs.13872
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