This Human D-Dimer (D2D) ELISA Kit is suitable for qualitatively determining D2D concentrations in multiple biological fluids, including human serum and plasma in vitro. D-dimer is the final degradation product of cross-linked fibrin generated during plasma coagulation. Yumeng Yao etc. demonstrated that D-dimer expression is commonly elevated in patients infected with SARS-CoV-2. D-dimer levels correlate with disease severity and are a reliable prognostic marker for in-hospital mortality in patients admitted for COVID-19.
This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human D2D in the sample. Standards and samples are respectively added to the microplate wells pre-coated with anti-human D2D antibody. Biotin-labeled D2D antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. D2D in the sample or standard is captured by the immobilized antibody and then binds to the biotinylated D2D monoclonal antibody. And the biotin on the biotinylated D2D antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of TMB substrate. The addition of the stop solution into the wells immediately turns the blue solution into a yellow one. The concentration of D2D in the samples is directly proportional to the OD value (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. See the manual for more information on validation.