Human Dickkopf-related protein 2(DKK2) ELISA kit

Code CSB-EL006921HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
dickkopf homolog 2 (Xenopus laevis)
Alternative Names
Dickkopf 2 ELISA Kit; Dickkopf gene 2 ELISA Kit; Dickkopf homolog 2 ELISA Kit; Dickkopf related protein 2 ELISA Kit; Dickkopf WNT signaling pathway inhibitor 2 ELISA Kit; Dickkopf-2 ELISA Kit; Dickkopf-related protein 2 ELISA Kit; Dickkopf2 ELISA Kit; DKK 2 ELISA Kit; Dkk-2 ELISA Kit; Dkk2 ELISA Kit; DKK2_HUMAN ELISA Kit; hDkk 2 ELISA Kit; hDkk-2 ELISA Kit; hDkk2 ELISA Kit
Abbreviation
DKK2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Stem Cells
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DKK2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %91
Range %88-98
1:2Average %86
Range %80-94
1:4Average %96
Range %92-102
1:8Average %95
Range %90-100
Recovery
The recovery of human DKK2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-97
EDTA plasma (n=4)9895-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.416 2.456 2.436 2.324
101.579 1.685 1.632 1.520
50.834 0.901 0.868 0.756
2.50.515 0.545 0.530 0.418
1.250.372 0.365 0.369 0.257
0.6250.266 0.255 0.261 0.149
0.3120.184 0.178 0.181 0.069
00.113 0.111 0.112  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human DKK2 ELISA Kit was designed for the quantitative measurement of Human DKK2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

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Target Background

Function
(From Uniprot)
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Gene References into Functions
  1. our findings demonstrate that DKK2 functions as a tumor suppressor through inhibiting cell proliferation and inducing apoptosis via regulating Wnt signaling during breast tumorigenesis. PMID: 28467796
  2. Knock down of the dickkopf WNT signaling pathway inhibitor 2 (DKK2) resulted in a significant suppression of HOXD10, HOXD11 and HOXD13 while over-expression of DKK2 and stimulation with factors of the WNT signaling pathway. PMID: 27363011
  3. epigenetic silencing of Wnt antagonists was associated with gastric carcinogenesis, and concurrent hypermethylation of SFRP2 and DKK2 could be a potential marker for a prognosis of poor overall survival. PMID: 28152305
  4. the genotyping and functional findings are consistent with the hypothesis that DKK2 is a tumor suppressor; by selectively retaining a transcriptionally inactive DKK2 allele, the reduction of DKK2 function results in unchecked Wnt/beta-catenin signaling, contributing to hepatocellular carcinoma oncogenesis. PMID: 27203079
  5. The present report suggested that DKK2 downregulation suppressed the proliferation and invasion of prostate cancer cells by inhibiting the Wnt/betacatenin signaling pathway. PMID: 27431620
  6. these findings reveal that the miR-187-5p-DKK2 pathway regulates Wnt/beta-catenin signaling, cell growth, and apoptosis. Our findings provide the first evidence of a role for miR-187-5p in promotion of B-cell ALL. PMID: 27296949
  7. Genetic variant in DKK2 gene is associated with gallbladder cancer. PMID: 26715268
  8. targeting of DKK2 by miR-154 leads to upregulation of beta-catenin expression and activation of the classical Wnt signaling pathway and cardiac fibroblasts. PMID: 27542661
  9. MiR-21 overexpression was significantly correlated with the DKK2-/beta-catenin- immunohistochemical phenotype. PMID: 23999978
  10. Data indicate that inhibiting dickkopf-related protein 2 (DKK2)with siDKK2 reduced the expression of DKK2, but had no effect on leptin expression. PMID: 25312721
  11. We found 1 DKK2 SNP to be significantly associated with alcohol-related harm in alcoholic subjects PMID: 24117450
  12. a new regulatory mechanism of miR-222 PMID: 23587485
  13. Our results provide strong evidence that DKK2 is a key player in Ewing sarcoma invasion and osteolysis and also in the differential phenotype of Ewing sarcoma cells. PMID: 23204234
  14. DKK2 may contribute to tumorigenesis in epithelial ovarian cancer(EOC) through the Wnt/beta-catenin signaling mechanisms. PMID: 22964660
  15. Elevated TGF-beta1 levels in osteoarthritic osteoblasts can stimulate DKK2 expression. PMID: 21312269
  16. In the study of the genetics of alcoholism, three SNPs in DKK2 (rs427983; rs419558; rs399087) demonstrated empirical significance. PMID: 20332099
  17. DKK2 is epigenetically silenced by methylation in higher grades and stages of renal cell carcinoma; results suggest that DKK2 inhibits renal cancer progression through apoptotic and cell cycle pathways PMID: 19755393
  18. Analysis of individual Dkk domains and chimeric Dkks shows that the carboxy-terminal domains of both Dkk1 & 2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. PMID: 12167704
  19. analysis of the structural basis of the interaction between Dkk and low density lipoprotein receptor-related protein (LRP) 5/6 PMID: 18524778

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Subcellular Location
Secreted.
Protein Families
Dickkopf family
Tissue Specificity
Expressed in heart, brain, skeletal muscle and lung.
Database Links

HGNC: 2892

OMIM: 605415

KEGG: hsa:27123

STRING: 9606.ENSP00000285311

UniGene: Hs.211869

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