Human Endothelin-2(EDN2)ELISA Kit

Code CSB-E16518h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human EDN2 ELISA Kit was designed for the quantitative measurement of Human EDN2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.688 pg/mL-300 pg/mL and the sensitivity is 1.172 pg/mL.

Target Name endothelin 2
Alternative Names EDN2 ELISA Kit; EDN2_HUMAN ELISA Kit; Endothelin 2 ELISA Kit; Endothelin-2 ELISA Kit; ET 2 ELISA Kit; ET-2 ELISA Kit; ET2 ELISA Kit; PPET2 ELISA Kit; Preproendothelin 2 ELISA Kit; Preproendothelin-2 ELISA Kit
Abbreviation EDN2
Uniprot No. P20800
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 4.688 pg/mL-300 pg/mL
Sensitivity 1.172 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EDN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 103
Range % 96-107
1:2 Average % 86
Range % 81-92
1:4 Average % 94
Range % 87-98
1:8 Average % 86
Range % 80-91
Recovery
The recovery of human EDN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 87-98
EDTA plasma (n=4) 94 90-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
300 1.832 1.988 1.910 1.802
150 1.214 1.192 1.203 1.095
75 0.760 0.721 0.741 0.633
37.5 0.442 0.451 0.447 0.339
18.75 0.287 0.276 0.282 0.174
9.375 0.212 0.217 0.215 0.107
4.688 0.155 0.160 0.158 0.050
0 0.107 0.108 0.108  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Endothelins are endothelium-derived vasoconstrictor peptides.
Gene References into Functions
  1. EDN2 expression in left internal mammary arteries depends on tissue harvesting technique. PMID: 24647318
  2. Overexpression of Edn2 in the mouse retina causes an excess of tip cells and inhibits vascular growth. Overexpression of Edn2 in the brain and skin had no effect, but vascular development was inhibited in midgestation mouse embryos. PMID: 24043815
  3. Endothelin 2 and endothelin receptor type B may be a regenerative pathway for central nervous system myelination and remyelination. PMID: 23518706
  4. Studies revealed hypermethylation of EDN2 and EDN3 genes in human primary colon cancers and in a panel of human colon cancer cell lines. Epigenetic inactivation of ET-2 and ET-3 occurs frequently in both rat and human colon cancers. PMID: 22865632
  5. plasma levels of big ET-2, and big ET-3 are markedly increased in patients with end stage renal disease on hemodialysis PMID: 22921304
  6. In granulosa lutein cells from women with polycystic ovary syndrome, endothelin-2 mRNA was reduced as compared with normally ovulating women PMID: 22727793
  7. although endothelin 2 does not appear to be an independent prognostic marker, there is evidence of a putative role in clear cell renal cell carcinoma progression PMID: 22047647
  8. Endothelin-2 is a macrophage chemoattractant. ET-2 expression by tumors may modulate the behavior of macrophages such that activated cells accumulate in areas of hypoxia. PMID: 12207323
  9. Endothelin-2 is a hypoxia-induced autocrine survival factor for breast tumor cells PMID: 12516960
  10. The EDN2 A985 allele, which is considered to be protective in cardiovascular disease, may be a risk factor for atrial fibrillation in patients with hypertrophic cardiomyopathy. PMID: 18037749

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Subcellular Location Secreted.
Protein Families Endothelin/sarafotoxin family
Tissue Specificity Expressed in lung, but not in placental stem villi vessels or cultured placental villi smooth muscle cells.
Database Links

HGNC: 3177

OMIM: 131241

KEGG: hsa:1907

STRING: 9606.ENSP00000361668

UniGene: Hs.1407

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