Product Details

Description

This Human EDN2 ELISA Kit was designed for the quantitative measurement of Human EDN2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.688 pg/mL-300 pg/mL and the sensitivity is 1.172 pg/mL.

Target Name

endothelin 2

Alternative Names

EDN2 ELISA Kit; EDN2_HUMAN ELISA Kit; Endothelin 2 ELISA Kit; Endothelin-2 ELISA Kit; ET 2 ELISA Kit; ET-2 ELISA Kit; ET2 ELISA Kit; PPET2 ELISA Kit; Preproendothelin 2 ELISA Kit; Preproendothelin-2 ELISA Kit

Abbreviation

EDN2

Uniprot No.

P20800

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

4.688 pg/mL-300 pg/mL

Sensitivity

1.172 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EDN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-107
1:2	Average %	86
	Range %	81-92
1:4	Average %	94
	Range %	87-98
1:8	Average %	86
	Range %	80-91

Recovery

The recovery of human EDN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	1.832	1.988	1.910	1.802
150	1.214	1.192	1.203	1.095
75	0.760	0.721	0.741	0.633
37.5	0.442	0.451	0.447	0.339
18.75	0.287	0.276	0.282	0.174
9.375	0.212	0.217	0.215	0.107
4.688	0.155	0.160	0.158	0.050
0	0.107	0.108	0.108

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Endothelins are endothelium-derived vasoconstrictor peptides.
Gene References into Functions	EDN2 expression in left internal mammary arteries depends on tissue harvesting technique. PMID: 24647318 Overexpression of Edn2 in the mouse retina causes an excess of tip cells and inhibits vascular growth. Overexpression of Edn2 in the brain and skin had no effect, but vascular development was inhibited in midgestation mouse embryos. PMID: 24043815 Endothelin 2 and endothelin receptor type B may be a regenerative pathway for central nervous system myelination and remyelination. PMID: 23518706 Studies revealed hypermethylation of EDN2 and EDN3 genes in human primary colon cancers and in a panel of human colon cancer cell lines. Epigenetic inactivation of ET-2 and ET-3 occurs frequently in both rat and human colon cancers. PMID: 22865632 plasma levels of big ET-2, and big ET-3 are markedly increased in patients with end stage renal disease on hemodialysis PMID: 22921304 In granulosa lutein cells from women with polycystic ovary syndrome, endothelin-2 mRNA was reduced as compared with normally ovulating women PMID: 22727793 although endothelin 2 does not appear to be an independent prognostic marker, there is evidence of a putative role in clear cell renal cell carcinoma progression PMID: 22047647 Endothelin-2 is a macrophage chemoattractant. ET-2 expression by tumors may modulate the behavior of macrophages such that activated cells accumulate in areas of hypoxia. PMID: 12207323 Endothelin-2 is a hypoxia-induced autocrine survival factor for breast tumor cells PMID: 12516960 The EDN2 A985 allele, which is considered to be protective in cardiovascular disease, may be a risk factor for atrial fibrillation in patients with hypertrophic cardiomyopathy. PMID: 18037749 Show More Hide All
Subcellular Location	Secreted.
Protein Families	Endothelin/sarafotoxin family
Tissue Specificity	Expressed in lung, but not in placental stem villi vessels or cultured placental villi smooth muscle cells.
Database Links	HGNC: 3177 OMIM: 131241 KEGG: hsa:1907 STRING: 9606.ENSP00000361668 UniGene: Hs.1407

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Check Code：

Code	CSB-E16518h
Size	96T,5×96T,10×96T
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Human Endothelin-2(EDN2)ELISA Kit

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