Recombinant Human Endothelin-2 (EDN2)

Code CSB-YP007401HU
MSDS
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Source Yeast
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Code CSB-EP007401HU
MSDS
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Source E.coli
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Code CSB-EP007401HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007401HU
MSDS
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Source Baculovirus
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Code CSB-MP007401HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EDN2
Uniprot No.
Alternative Names
EDN2; EDN2_HUMAN; Endothelin 2; Endothelin-2; ET 2; ET-2; ET2; PPET2; Preproendothelin 2; Preproendothelin-2
Species
Homo sapiens (Human)
Expression Region
49-69
Target Protein Sequence
CS CSSWLDKECV YFCHLDIIW
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Endothelins are endothelium-derived vasoconstrictor peptides.
Gene References into Functions
  1. EDN2 expression in left internal mammary arteries depends on tissue harvesting technique. PMID: 24647318
  2. Overexpression of Edn2 in the mouse retina causes an excess of tip cells and inhibits vascular growth. Overexpression of Edn2 in the brain and skin had no effect, but vascular development was inhibited in midgestation mouse embryos. PMID: 24043815
  3. Endothelin 2 and endothelin receptor type B may be a regenerative pathway for central nervous system myelination and remyelination. PMID: 23518706
  4. Studies revealed hypermethylation of EDN2 and EDN3 genes in human primary colon cancers and in a panel of human colon cancer cell lines. Epigenetic inactivation of ET-2 and ET-3 occurs frequently in both rat and human colon cancers. PMID: 22865632
  5. plasma levels of big ET-2, and big ET-3 are markedly increased in patients with end stage renal disease on hemodialysis PMID: 22921304
  6. In granulosa lutein cells from women with polycystic ovary syndrome, endothelin-2 mRNA was reduced as compared with normally ovulating women PMID: 22727793
  7. although endothelin 2 does not appear to be an independent prognostic marker, there is evidence of a putative role in clear cell renal cell carcinoma progression PMID: 22047647
  8. Endothelin-2 is a macrophage chemoattractant. ET-2 expression by tumors may modulate the behavior of macrophages such that activated cells accumulate in areas of hypoxia. PMID: 12207323
  9. Endothelin-2 is a hypoxia-induced autocrine survival factor for breast tumor cells PMID: 12516960
  10. The EDN2 A985 allele, which is considered to be protective in cardiovascular disease, may be a risk factor for atrial fibrillation in patients with hypertrophic cardiomyopathy. PMID: 18037749

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Subcellular Location
Secreted.
Protein Families
Endothelin/sarafotoxin family
Tissue Specificity
Expressed in lung, but not in placental stem villi vessels or cultured placental villi smooth muscle cells.
Database Links

HGNC: 3177

OMIM: 131241

KEGG: hsa:1907

STRING: 9606.ENSP00000361668

UniGene: Hs.1407

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