Human Epidermal growth factor-like protein 6(EGFL6) ELISA kit

Instructions
Code CSB-EL007475HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name EGF-like-domain, multiple 6
Alternative Names EGF like domain multiple 6 ELISA Kit; EGF repeat containing protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; EGF-like-domain multiple 6 ELISA Kit; egfl6 ELISA Kit; EGFL6_HUMAN ELISA Kit; epidermal growth factor like protein 6 ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; MAEG ELISA Kit; MAM and EGF domain containing ELISA Kit; MAM and EGF domains containing gene protein ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; W80 ELISA Kit
Abbreviation EGFL6
Uniprot No. Q8IUX8
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 104
Range % 97-108
1:2 Average % 101
Range % 93-105
1:4 Average % 97
Range % 89-101
1:8 Average % 104
Range % 94-109
Recovery
The recovery of human EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 83-94
EDTA plasma (n=4) 102 95-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 1.972 1.918 1.945 1.800
2500 1.400 1.439 1.420 1.275
1250 1.072 1.025 1.049 0.904
625 0.762 0.734 0.748 0.603
312 0.521 0.529 0.525 0.380
156 0.348 0.357 0.353 0.208
78 0.271 0.283 0.277 0.132
0 0.148 0.142 0.145  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly (By similarity).
Gene References into Functions
  1. In conclusion, our results suggest that plasma level of EGFL6 may be useful to assess disease progression, and especially advanced T status and higher TNM stage in patients with oral squamous cell carcinoma. PMID: 28539817
  2. EGFL6 is an angiocrine and tumor cell factor that promotes tumorigenesis via SHP2-mediated signaling. EGFL6 stimulates ALDH+ ovarian cancer stem cell to migrate and undergo asymmetric division. PMID: 27803106
  3. EGFL6 was overexpressed in benign meningioma tissues and serum. PMID: 23285163
  4. results indicate that human EGFL6 is a paracrine/autocrine growth factor of adipose tissue up-regulated in obesity and potentially involved in the process of adipose tissue expansion and the development of obesity PMID: 20574786
  5. There is an absence of GNAS and EGFL6 mutations in common human cancers. PMID: 18038328

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Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Protein Families Nephronectin family
Database Links

HGNC: 3235

OMIM: 300239

KEGG: hsa:25975

STRING: 9606.ENSP00000369976

UniGene: Hs.12844

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