Human Fibulin-7(FBLN7) ELISA kit

Code CSB-EL008455HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
fibulin 7
Alternative Names
FBLN7 ELISA Kit; FBLN7_HUMAN ELISA Kit; FIBL-7 ELISA Kit; Fibl7 ELISA Kit; Fibulin-7 ELISA Kit; TM14 ELISA Kit
Abbreviation
FBLN7
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FBLN7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 87-99
1:2 Average % 95
Range % 87-100
1:4 Average % 92
Range % 87-96
1:8 Average % 97
Range % 91-101
Recovery
The recovery of human FBLN7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 87-97
EDTA plasma (n=4) 91 84-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.435 2.322 2.379 2.287
800 1.803 1.746 1.775 1.683
400 1.162 1.123 1.143 1.051
200 0.549 0.535 0.542 0.450
100 0.332 0.314 0.323 0.231
50 0.208 0.203 0.206 0.114
25 0.114 0.108 0.111 0.019
0 0.093 0.091 0.092  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human FBLN7 ELISA Kit was designed for the quantitative measurement of Human FBLN7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)
An adhesion molecule that interacts with extracellular matrix molecules in developing teeth and may play important roles in differentiation and maintenance of odontoblasts as well as in dentin formation.
Gene References into Functions
  1. Fbln7-C terminal fragment inhibited the HUVEC tube formation and the vessel sprouting in aortic ring assays. PMID: 24480309
  2. Dysregulation of fibulin expression by anti-Ro/SSA antibodiess may contribute to disorganization of the extracellular environment and thus cause injury to the salivary gland architecture and functionality observed in Sjogren syndrome PMID: 19229767
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Fibulin family
Database Links

HGNC: 26740

OMIM: 611551

KEGG: hsa:129804

STRING: 9606.ENSP00000331411

UniGene: Hs.437696

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