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Human Folic acid(FA) ELISA Kit

Instructions
Code CSB-E17109h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Human Folic acid (FA) ELISA Kit manufactured by CUSABIO is a ready-to-use ELISA kit for the quantification of FA in the human serum and plasma. It has been validated in various performances, including specificity, sensitivity, linearity, precision, recovery, and lot-to-lot consistency. Based on the competitive inhibition enzyme immunoassay technique, FA in the samples or standards and HRP-conjugated FA with pre-coated FA antibody. Following the addition of TMB, the color develops in opposite to the amount of FA in the sample.

FA is a B vitamin found in many vitamin supplements and fortified foods. It's the synthetic form of folate, a naturally occurring B vitamin. The human body uses FA to make new cells and produce DNA. FA is essential for normal growth and development throughout life. FA is particularly important for women who get pregnant or are pregnant. It is recommended to take FA before and during pregnancy. FA protects intrauterine babies from serious birth defects.
Target Name Folic acid(FA)
Alternative Names N/A
Abbreviation FA
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 12.5 pg/mL-200 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:20 Average % 89  
Range % 84-93  
1:40 Average % 93  
Range % 89-97  
1:80 Average % 95  
Range % 90-100  
1:160 Average % 84  
Range % 80-88  
Recovery
The recovery of human FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 94-105  
EDTA plasma (n=4) 95 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
200 0.093 0.090 0.092    
100 0.227 0.208 0.218    
50 0.378 0.367 0.373    
25 0.618 0.637 0.628    
12.5 0.986 1.003 0.995    
0 1.485 1.429 1.457    
Materials provided
  • A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human FA antibody.
  • Standard (10 x concentrate)(1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • HRP-conjugated FA (100 x concentrate) (1 x 6oμl)
  • HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated FA antibody solution.
  • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
  • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
  • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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■ Q&A
Q:

We are looking for Elisa kit for human folic acid, detection range: 310-1000 nmol/L.
Material: erythrocytes (not full blood)
Please tell me is the result obtained from your kit can be comparable to the microbiological reference method?

A:
Thank you for your enquiry!Currently we just have this kit, and the detailed information, pls check the manual.
The theory of different detection methods are different.Since we have no information which is comparable to the microbiological reference method.
Pls just take your own actual test result.
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