Bovine folic acid,FA ELISA Kit

Code CSB-E13074B
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation FA
Protein Biological Process 1 Metabolism
Target Name folic acid,FA
Alias N/A
Species Bos taurus (Bovine)
Sample Types serum, plasma,tissue homogenates
Detection Range 0.39 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 95  
Range % 90-100  
1:40 Average % 93  
Range % 89-97  
1:80 Average % 100  
Range % 95-104  
1:160 Average % 88  
Range % 84-92  
Recovery
The recovery of bovine FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-97  
EDTA plasma (n=4) 98 93-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
500 0.158 0.148 0.153    
250 0.293 0.278 0.286    
125 0.477 0.506 0.492    
62.5 0.772 0.744 0.758    
31.2 1.040 0.986 1.013    
15.6 1.545 1.513 1.529    
7.8 1.667 1.701 1.684    
0 1.785 1.747 1.766    
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Q&A and Customer Reviews

Q&A
Q:

Can you pls provide me below information: 1) Is the standard a recombinant protein and is baculovirus used to produce it?
2) What is the composition of the Stop Solution?
3) What preservatives are used in the kit and what specific components contain them?

A:
Thanks for your inquiry. For CSB-E13074B:
Standard is purified small molecule.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).Pls let me know if you have any further questions. Thank you.
Customer Reviews

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