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Mouse Folic acid,FA ELISA Kit

Citations (2) Protocol
Code CSB-E08758m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Folic acid,FA
Alternative Names
N/A
Abbreviation
FA
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
0.195 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 97
Range % 90-105
1:2 Average % 89
Range % 83-96
1:4 Average % 92
Range % 84-99
1:8 Average % 91
Range % 85-99
Recovery
The recovery of mouse FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 87-100
EDTA plasma (n=4) 95 89-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
50 0.321 0.332 0.327
12.5 0.512 0.537 0.525
3.12 0.826 0.851 0.839
0.78 1.175 1.153 1.164
0.195 1.655 1.632 1.644
0 1.985 1.948 1.967
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse Folic acid (FA) ELISA Kit was designed for the quantitative measurement of Mouse Folic acid (FA) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.195 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

Citations

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■ Q&A
Q:

I would like to know what is the format of this assay. Is it a 1 96 well plate or the plate can be divided into strips (8x12)?
Whatever the answer is – is it true for all your ELISA kits?

A:
Thanks for your inquiry.
Most of our 96T kits is 8wells× 12 strips, which can be divided into strips.
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