Mouse Folic acid,FA ELISA Kit

Instructions
Code CSB-E08758m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name Folic acid,FA
Alternative Names N/A
Abbreviation FA
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 0.195 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 90-105
1:2 Average % 89
Range % 83-96
1:4 Average % 92
Range % 84-99
1:8 Average % 91
Range % 85-99
Recovery
The recovery of mouse FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 87-100
EDTA plasma (n=4) 95 89-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
50 0.321 0.332 0.327
12.5 0.512 0.537 0.525
3.12 0.826 0.851 0.839
0.78 1.175 1.153 1.164
0.195 1.655 1.632 1.644
0 1.985 1.948 1.967
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

I would like to know what is the format of this assay. Is it a 1 96 well plate or the plate can be divided into strips (8x12)?
Whatever the answer is – is it true for all your ELISA kits?

A:
Thanks for your inquiry.
Most of our 96T kits is 8wells× 12 strips, which can be divided into strips.

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  Email: support@cusabio.com
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