Rat Folic acid,FA ELISA Kit

Code CSB-E08757r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
Folic acid,FA
Alternative Names
N/A
Abbreviation
FA
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma
Detection Range
19.5 pg/mL-5000 pg/mL
Sensitivity
19.5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat folic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 86-104  
1:2 Average % 92  
Range % 83-99  
1:4 Average % 96  
Range % 87-98  
1:8 Average % 94  
Range % 80-98  
Recovery
The recovery of rat folic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 86-102  
EDTA plasma (n=4) 94 88-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
5000 0.324 0.326 0.325    
1250 0.514 0.528 0.521    
312 0.817 0.814 0.816    
78 1.281 1.293 1.287    
19.5 1.674 1.635 1.655    
0 1.948 1.966 1.957    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days
Description

This Rat Folic acid (FA) ELISA Kit was designed for the quantitative measurement of Rat Folic acid (FA) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 19.5 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

Customer Reviews and Q&A

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 Q&A
Q:

Regarding CSB-E08757r-1plate-we would like to know the origin of the standard Folic Acid? Is it derived from rat or baculovirus etc?

A:
Thanks for your inquiry.
The standard is purified small molecule. Sorry we can not disclose the specific origin. Hope you could understand.
Q:

Sorry to bothering you, and I have questions about your product CSB-E08757r.
1) Could you let us know the chemical name and concentration of acid in Stop Solution?
2) Could you let us know whether any preservatives are contained in each component?”.

A:
Thanks for your inquiry.
CSB-E08757r
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
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