Human Glutathione peroxidase 3(GPX3) ELISA kit

Code CSB-E09496h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P22352
Lead Time 3-5 working days
Abbreviation GPX3
Target Name glutathione peroxidase 3
Alias GPx-P, GSHPx-3, GSHPx-P, extracellular glutathione peroxidase|glutathione peroxidase 3
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 μIU/mL-1000 μIU/mL
Sensitivity 3.9 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3 UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
HGNC 4555
RGD 69224
MGI 105102
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GPX3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %93
Range %89-95
1:2000Average %104
Range %100-107
1:4000Average %92
Range %89-96
1:8000Average %98
Range %95-103
Recovery
The recovery of human GPX3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-98
EDTA plasma (n=4)9694-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
µIU/mlOD1OD2AverageCorrected
10002.630 2.566 2.598 2.458
5002.330 2.224 2.277 2.137
2501.736 1.704 1.720 1.580
1251.000 1.052 1.026 0.886
62.50.622 0.608 0.615 0.475
31.250.351 0.369 0.360 0.220
15.60.234 0.222 0.228 0.088
00.142 0.138 0.140  
References
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Function Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
Subcellular Location Secreted
Protein Families Glutathione peroxidase family
Tissue Specificity Secreted in plasma.
Database Links

HGNC: 4555

OMIM: 138321

KEGG: hsa:2878

STRING: 9606.ENSP00000373477

UniGene: Hs.386793

Pathway Th17 cell differentiation

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