Human Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

Code CSB-EL018086HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
phospholipase A2, group XIIA
Alternative Names
EC 3.1.1.4 ELISA Kit; Group XII secreted phospholipase A2 ELISA Kit; Group XIIA secreted phospholipase A2 ELISA Kit; Group XIIA secretory phospholipase A2 ELISA Kit; Group XIIA secretory phospholipase A2 precursor ELISA Kit; GXII ELISA Kit; GXII sPLA2 ELISA Kit; PG12A_HUMAN ELISA Kit; phosphatidylcholine 2 acylhydrolase 12A ELISA Kit; Phosphatidylcholine 2 acylhydrolase GXII ELISA Kit; Phosphatidylcholine 2-acylhydrolase 12A ELISA Kit; Phospholipase A2 group XIIA ELISA Kit; PLA2G12 ELISA Kit; Pla2g12a ELISA Kit; ROSSY ELISA Kit; sPLA2 XII ELISA Kit; sPLA2-XII ELISA Kit
Abbreviation
PLA2G12A
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.7 pg/mL-300 pg/mL
Sensitivity
1.2 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:20 Average % 93    
Range % 90-96    
1:40 Average % 107    
Range % 103-110    
1:80 Average % 86    
Range % 83-90    
1:160 Average % 101    
Range % 98-106    
Recovery
The recovery of human PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 85 80-90    
EDTA plasma (n=4) 101 97-106    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average      
300 0.236 0.230 0.233      
150 0.386 0.391 0.389      
75 0.589 0.592 0.591      
37.5 0.847 0.834 0.841      
18.75 1.076 1.100 1.088      
9.4 1.534 1.502 1.518      
4.7 1.952 1.991 1.972      
0 2.345 2.401 2.373      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PLA2G12A ELISA Kit was designed for the quantitative measurement of Human PLA2G12A protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.2 pg/mL.

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Target Background

Function
(From Uniprot)
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine.
Gene References into Functions
  1. The study demonstrated that PLA2G12A SNPs or haplotypes might influence the susceptibility to schizophrenia in the Han Chinese population from Northeast China. PMID: 27434078
  2. cellular arachidonate (AA) release and prostaglandin (PG) production are regulated by novel classes of secretory phospholipase A(2)s (sPLA(2)s), groups III and XII PMID: 12522102
Subcellular Location
Secreted. Cytoplasm.
Protein Families
Phospholipase A2 family
Tissue Specificity
Abundantly expressed in heart, skeletal muscle, kidney, liver and pancreas.
Database Links

HGNC: 18554

OMIM: 611652

KEGG: hsa:81579

STRING: 9606.ENSP00000243501

UniGene: Hs.389452

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