Human Growth hormone variant(GH2) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Alternative Names

GH2 ELISA Kit; Growth hormone variant ELISA Kit; GH-V ELISA Kit; Growth hormone 2 ELISA Kit; Placenta-specific growth hormone ELISA Kit

Abbreviation

GH2

Uniprot No.

P01242

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

133 pg/mL-4000 pg/mL

Sensitivity

33 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-98
1:2	Average %	90
	Range %	85-97
1:4	Average %	94
	Range %	90-101
1:8	Average %	94
	Range %	86-102

Recovery

The recovery of human GH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	93	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.531	2.447	2.489	2.443
1666.7	1.295	1.342	1.319	1.273
666.7	0.628	0.607	0.618	0.572
266.7	0.285	0.306	0.296	0.250
133	0.163	0.152	0.158	0.112
0	0.045	0.047	0.046

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human GH2 ELISA Kit was designed for the quantitative measurement of Human GH2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 133 pg/mL-4000 pg/mL and the sensitivity is 33 pg/mL.

Target Background

Function
(From Uniprot)

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Gene References into Functions

Type 1 diabetic women delivering large for gestational age infants had lower placental GH levels in early pregnancy. PMID: 26589570
genetic association studies in populations in Estonia, Czech Republic, and Bashkortostan/Russia: Data suggest an SNP in GH2 (rs2006123) is associated with adult height (not body mass index); A-allele is associated with taller stature. [META-ANALYSIS] PMID: 24035309
Maternal serum PGH at 11-13 weeks' gestation is unlikely to be a useful biochemical marker for early prediction of small for gestational age neonates. PMID: 22489624
results are consistent with a pleiotropic effect of placental hGH/CSH genes at the maternal-fetal interface relating to the regulation of fetal growth and the risk of affected maternal metabolism. PMID: 22387044
These results suggest that the normal PGH-IGF-I-IGFBP3 axis in pregnancy is abnormal in Type 1 Diabetic pregnancies. PMID: 22363400
early first trimester maternal serum marker for Down syndrome PMID: 19844941
The hGH-V promoter activity and the endogenous mRNA expression in human placental choriocarcinoma cell line JAR in the presence of glucose and demethylating genome conditions, were compared. PMID: 20005926
GH-V plays a major role in affecting target cells expressing the GH receptor, thus potentially exerting significant GH-like effects on maternal physiology during pregnancy. PMID: 12213191
Data show that improved binding affinity of human growth hormone (hGH(v)) results from numerous small contributions distributed broadly over the interface. PMID: 12946357
Using a mutagenesis-scanning analysis of 81 single and 32 pairwise double mutations, it is shown that the GHv hormone's two spatially distal receptor binding sites (Site1 and Site2) are allosterically coupled PMID: 15563602
results offer the first evidence for a placental role of human placental growth hormone(hPGH) and suggest an autocrine/paracrine role of hPGH in the regulation of trophoblast invasion PMID: 15718272
PGH may play a role in the mechanisms of disease in preeclampsia and fetal growth restriction. PMID: 17701665
Placental growth hormone (PGH) secretion by BeWo cells is regulated by hormonal factors and adipokines. Elevated insulin and leptin levels might down-regulate PGH secretion. PMID: 18356738

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Subcellular Location

Secreted.

Protein Families

Somatotropin/prolactin family

Tissue Specificity

Expressed in the placenta.

Database Links

HGNC: 4262

OMIM: 139240

KEGG: hsa:2689

STRING: 9606.ENSP00000333157

UniGene: Hs.406754

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Code	CSB-EL009408HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.