Human Homocysteine(HCY)ELISA Kit

Instructions
Code CSB-E13814h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Homocysteine(HCY)
Alternative Names N/A
Abbreviation Hcy
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, tissue homogenates
Detection Range 0.78 nmol/mL-50 nmol/mL
Sensitivity 0.195 nmol/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HCY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-90  
1:2 Average % 98  
Range % 91-105  
1:4 Average % 89  
Range % 84-96  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human HCY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 91-101  
EDTA plasma (n=4) 92 89-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nmol/ml OD1 OD2 Average Corrected  
50 2.082 2.156 2.119 1.997  
25 1.456 1.489 1.473 1.351  
12.5 0.941 0.968 0.955 0.833  
6.25 0.583 0.597 0.590 0.468  
3.125 0.342 0.367 0.355 0.233  
1.56 0.248 0.261 0.255 0.133  
0.78 0.192 0.198 0.195 0.073  
0 0.121 0.123 0.122    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Customer Reviews
  • Sample type: Serum
    Sample species: Human
    Review: The test values for Homocysteine came out as expected. They were normal group of patients and as such, the results were expected to be within range.

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