Human Hyperglycosylated Chorionic Gonadotropin(hCG) ELISA Kit

Instructions
Code CSB-E15803h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Hyperglycosylated Chorionic Gonadotropin(hCG)
Alternative Names N/A
Abbreviation hCG
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, cell culture supernates
Detection Range 0.67 mIU/mL-20 mIU/mL
Sensitivity 0.167 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human hCG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 91-99  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 96  
Range % 90-100  
1:8 Average % 95  
Range % 89-101  
Recovery
The recovery of human hCG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 91-103  
EDTA plasma (n=4) 94 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average Corrected  
20 2.542 2.539 2.541 2.493  
8.3 1.282 1.276 1.279 1.231  
3.3 0.642 0.634 0.638 0.590  
1.3 0.288 0.285 0.287 0.239  
0.67 0.156 0.149 0.153 0.105  
0 0.049 0.047 0.048    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Can you confirm the assay is specific for hyperglycosylated HCG?

A:
Thanks for your inquiry.
Yes, the kit CSB-E15803h is specifically for Hyperglycosylated Chorionic Gonadotropin(hCG).Pls let me know if you have any further questions. Thank you.

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