Human Hyperglycosylated Chorionic Gonadotropin(hCG) ELISA Kit

Code CSB-E15803h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Hyperglycosylated Chorionic Gonadotropin(hCG)
Alternative Names
N/A
Abbreviation
hCG
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, cell culture supernates
Detection Range
0.67 mIU/mL-20 mIU/mL
Sensitivity
0.167 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human hCG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 91-99  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 96  
Range % 90-100  
1:8 Average % 95  
Range % 89-101  
Recovery
The recovery of human hCG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 91-103  
EDTA plasma (n=4) 94 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average Corrected  
20 2.542 2.539 2.541 2.493  
8.3 1.282 1.276 1.279 1.231  
3.3 0.642 0.634 0.638 0.590  
1.3 0.288 0.285 0.287 0.239  
0.67 0.156 0.149 0.153 0.105  
0 0.049 0.047 0.048    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human hCG ELISA Kit was designed for the quantitative measurement of Human hCG protein in serum, plasma, urine, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.67 mIU/mL-20 mIU/mL and the sensitivity is 0.167 mIU/mL.

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 Q&A
Q:

Can you confirm the assay is specific for hyperglycosylated HCG?

A:
Thanks for your inquiry.
Yes, the kit CSB-E15803h is specifically for Hyperglycosylated Chorionic Gonadotropin(hCG).Pls let me know if you have any further questions. Thank you.
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