Human Immunoglobulin J chain(IGJ) ELISA kit

Code CSB-E09237h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
Alternative Names
IGCJ ELISA Kit; IGJ_HUMAN ELISA Kit; Immunoglobulin J chain ELISA Kit; Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides ELISA Kit; immunoglobulin joining chain ELISA Kit; JCH ELISA Kit; JCHAIN ELISA Kit; Joining chain of multimeric IgA and IgM ELISA Kit
Abbreviation
IGJ
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, saliva, urine, tear, breast milk
Detection Range
10 ng/mL-40000 ng/mL
Sensitivity
10 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays):CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IGJ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1000 Average % 89    
Range % 81-99    
1:2000 Average % 95    
Range % 83-99    
1:4000 Average % 95    
Range % 90-102    
1:8000 Average % 90    
Range % 85-100    
Recovery
The recovery of human IGJ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 92 85-99    
EDTA plasma (n=4) 95 90-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
40000 2.257 2.242 2.250 2.162    
10000 2.154 2.138 2.146 2.058    
2500 1.892 1.864 1.878 1.790    
625 1.252 1.249 1.251 1.163    
156 0.587 0.598 0.593 0.505    
39 0.282 0.288 0.285 0.197    
10 0.141 0.139 0.140 0.052    
0 0.086 0.090 0.088      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human IGJ ELISA Kit was designed for the quantitative measurement of Human IGJ protein in serum, plasma, saliva, urine, tear, breast milk. It is a Sandwich ELISA kit, its detection range is 10 ng/mL-40000 ng/mL and the sensitivity is 10 ng/mL.

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Target Background

Function
(From Uniprot)
Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to secretory component.
Gene References into Functions
  1. TLR2 ligands induce CCR9 and CCR10 expression by circulating B-cells and increase their chemotaxis. TLR2 stimulation also induced J chain and IgA production demonstrating the induction of mucosal-like antibody secreting cells. PMID: 20947433
Subcellular Location
Secreted.
Database Links

HGNC: 5713

OMIM: 147790

KEGG: hsa:3512

STRING: 9606.ENSP00000254801

UniGene: Hs.643431

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