Rabbit Immunoglobulin M,IgM ELISA Kit

Instructions
Code CSB-E06950Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Immunoglobulin M,IgM
Abbreviation IgM
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 15.6 ng/mL-4000 ng/mL
Sensitivity 4 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 96
Range % 87-105
1:2000 Average % 92
Range % 85-100
1:4000 Average % 93
Range % 89-100
1:8000 Average % 94
Range % 85-99
Recovery
The recovery of rabbit IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 93 87-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
4000 0.225 0.218 0.222
1000 0.351 0.345 0.348
250 0.623 0.617 0.62
62.5 1.127 1.139 1.133
15.6 2.226 2.241 2.234
0 2.911 2.984 2.948
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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