Rabbit Immunoglobulin M,IgM ELISA Kit

Code CSB-E06950Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
Immunoglobulin M,IgM
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma
Detection Range
15.6 ng/mL-4000 ng/mL
4 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 96
Range % 87-105
1:2000 Average % 92
Range % 85-100
1:4000 Average % 93
Range % 89-100
1:8000 Average % 94
Range % 85-99
The recovery of rabbit IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 93 87-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
4000 0.225 0.218 0.222
1000 0.351 0.345 0.348
250 0.623 0.617 0.62
62.5 1.127 1.139 1.133
15.6 2.226 2.241 2.234
0 2.911 2.984 2.948
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Rabbit IgM ELISA Kit was designed for the quantitative measurement of Rabbit IgM protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 15.6 ng/mL-4000 ng/mL and the sensitivity is 4 ng/mL.

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