Human Inositol oxygenase(MIOX) ELISA kit

Code CSB-EL013833HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
myo-inositol oxygenase
Alternative Names
0610009I10Rik ELISA Kit; AI314022 ELISA Kit; Aldehyde reductase-like 6 ELISA Kit; ALDRL6 ELISA Kit; C85427 ELISA Kit; Inositol oxygenase ELISA Kit; Kidney-specific protein 32 ELISA Kit; KSP32 ELISA Kit; MGC90217 ELISA Kit; MI oxygenase ELISA Kit; miox ELISA Kit; MIOX_HUMAN ELISA Kit; Myo-inositol oxygenase ELISA Kit; Renal-specific oxidoreductase ELISA Kit; RSOR ELISA Kit
Abbreviation
MIOX
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
18.75 pg/mL-1200 pg/mL
Sensitivity
4.7 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MIOX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 84-92
1:2 Average % 102
Range % 99-105
1:4 Average % 86
Range % 81-90
1:8 Average % 98
Range % 94-102
Recovery
The recovery of human MIOX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 89-100
EDTA plasma (n=4) 91 88-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1200 2.443 2.378 2.411 2.217
600 2.045 2.056 2.051 1.857
300 1.577 1.398 1.488 1.294
150 0.984 0.987 0.986 0.792
75 0.641 0.638 0.640 0.446
37.5 0.434 0.445 0.440 0.246
18.75 0.334 0.329 0.332 0.138
0 0.195 0.193 0.194  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human MIOX ELISA Kit was designed for the quantitative measurement of Human MIOX protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

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Target Background

Gene References into Functions
  1. Our results suggest that serum MIOX levels do not change in polycystic ovary syndrome. It was, therefore, concluded that myoinositol deficiency observed in PCOS was not related to the level of MIOX enzyme which cleaves myoinositol. PMID: 29187000
  2. these findings highlight the epigenetic regulation of MIOX in the pathogenesis of diabetic tubulopathy. PMID: 28208054
  3. Data suggest that MIOX expression/activity is up-regulated in kidneys tubules during oxidative stress (as in obesity and diabetic nephropathy); up-regulation of MIOX activity is related to phosphorylation of serine/threonine residues. PMID: 26578517
  4. results demonstrated that the polymorphism (rs761745) in the promoter region of myo-inositol oxygenase(MIOX) gene may be associated with the development of type 1 diabetes mellitus in our studied population PMID: 19896870
  5. Molecular cloning and characterization of myo-inositol oxygenase as a 33kDa protein. PMID: 15504367
  6. expression of MIOX is up-regulated by a positive feedback mechanism where xylitol, one of the products of MI catabolism via the glucuronate-xylulose pathway, induces an overexpression of MIOX PMID: 15778219
  7. myo-inositol oxygenase (MIOX) PMID: 11716759

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Subcellular Location
Cytoplasm.
Protein Families
Myo-inositol oxygenase family
Tissue Specificity
Kidney specific.
Database Links

HGNC: 14522

OMIM: 606774

KEGG: hsa:55586

STRING: 9606.ENSP00000216075

UniGene: Hs.129227

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