Human Interferon alpha-inducible protein 27, mitochondrial(IFI27) ELISA kit

Instructions
Code CSB-EL011009HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interferon, alpha-inducible protein 27
Alternative Names 2310061N23Rik ELISA Kit; FAM 14D ELISA Kit; FAM14D ELISA Kit; IFI 27 ELISA Kit; IFI27 ELISA Kit; IFI27_HUMAN ELISA Kit; Interferon alpha induced 11.5 kDa protein ELISA Kit; Interferon alpha inducible protein 27 ELISA Kit; Interferon alpha-induced 11.5 kDa protein ELISA Kit; Interferon alpha-inducible protein 27 ELISA Kit; interferon alpha-inducible protein 27, mitochondrial ELISA Kit; Interferon inducible protein 27 ELISA Kit; interferon-stimulated gene 12 ELISA Kit; Interferon-stimulated gene 12a protein ELISA Kit; ISG 12 ELISA Kit; ISG12 ELISA Kit; ISG12(a) ELISA Kit; ISG12(a) protein ELISA Kit; ISG12A ELISA Kit; mitochondrial ELISA Kit; p27 ELISA Kit
Abbreviation IFI27
Uniprot No. P40305
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 28 pg/mL-1800 pg/mL
Sensitivity 7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IFI27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-99
1:2Average %86
Range %82-93
1:4Average %94
Range %89-98
1:8Average %92
Range %85-97
Recovery
The recovery of human IFI27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8784-90
EDTA plasma (n=4)9995-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
18002.630 2.458 2.544 2.411
9002.126 2.222 2.174 2.041
4501.394 1.511 1.453 1.320
2250.768 0.785 0.777 0.644
1120.512 0.557 0.535 0.402
560.342 0.321 0.332 0.199
280.238 0.245 0.242 0.109
00.134 0.131 0.133  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Promotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9.
Gene References into Functions
  1. High IFI27 expression is associated with invasion in oral squamous cell carcinoma. PMID: 29580248
  2. NEAT1 and the coding gene IFI27 were highly co-expressed and negatively correlated with dengue severity. Monitoring NEAT1and IFI27 expression in PBMC may be useful in understanding dengue virus-induced disease progression. PMID: 29031635
  3. IFI27 was expressed in Influenza patients but not in Bacterial Respiratory Infection. IFI27 displayed 88% diagnostic accuracy (AUC) and 90% specificity in discriminating between Influenza and Bacterial Infections. PMID: 28619954
  4. ISG12a inhibited HCV replication and potentiated the anti-HCV activity of IFN-alpha possibly through induced production of type I IFNs and activation of Jak/STAT signaling pathway independent of autophagy and cell apoptosis. PMID: 27777077
  5. the ISG12 family of proteins has an important role for the apoptotic properties induced by type 1 interferon. PMID: 27673746
  6. IFI27 mRNA level is reduced in peripheral blood mononuclear cells of immunoglobulin A nephropathy and membranous nephropathy patients. PMID: 27100186
  7. IFI27 is involved in proliferation of skin keratinocytes both in vitro and in vivo. PMID: 25664647
  8. Data indicate that AKT control TRAIL resistance of cancer cells through downregulation of interferon stimulated gene 12a (ISG12a) by MicroRNA-942 (miR-942). PMID: 24970806
  9. We found that IFI27 is overexpressed in ovarian cancer and associated with patients' survival. PMID: 25103640
  10. MiR-942 regulates hepatitis c virus-induced apoptosis of human hepatocytes by targeting ISG12a. PMID: 24727952
  11. These results suggest that high basal ISG12a may inhibit Newcastle disease virus replication and oncolysis, while low basal ISG12a may allow sufficient Newcastle disease virus replication for induction of ISG12a. PMID: 24999841
  12. Alterations in mechanisms associated with immune response and mitochondrial function that explain the up-regulation of IFI27 may provide an understanding of the pathways related to the intensification of fatigue during localized radiation therapy. PMID: 23959120
  13. After inhibiting the autophagy, the expression of interferon b1 and IFI27 were decreased. PMID: 23383510
  14. Patients with atypical teratoid/rhabdoid tumors show high p27 expression when angiogenesis and density of newly formed blood vessels were investigated. PMID: 22309998
  15. interferon alpha-inducible protein 27 (IFI27) transcription stimulation was weaker in leiomyoma than myometrial cells PMID: 22320196
  16. IFI27 may be a novel biomarker of disease activity and tumor burden in patients with chronic myeloproliferative neoplasms PMID: 21447007
  17. We identify IFI27, CCL2 and CXCL10 as sensitive biomarkers for the response of multiple sclerosis patients to IFN-beta. PMID: 19558503
  18. Assessment of p21, p27, Bax, and cyclin E expression in tumor tissues have been reported to be useful as prognostic factors in head and neck squamous cell carcinoma. PMID: 14719078
  19. Upregulated in whole blood of infants hospitalised with respiratory syncytial virus (RSV), subtype B, bronchiolitis. PMID: 17166282
  20. REsults describe the differential regulation of the interferon induced gene ISG12A by serum from healthy and preeclamptic pregnancies. PMID: 18329281
  21. data suggest that ISG12a contributes to IFN-dependent perturbation of normal mitochondrial function, thus adding ISG12a to a growing list of IFN-induced proteins that impact cellular apoptosis. PMID: 18330707
  22. five genes (TNFSF10/TRAIL, IL1RN, IFI27, GZMB, and CCR5) were upregulated and three genes (CLK1, TNFAIP3 and BTG1) were downregulated in at least three out of four subpopulations during acute GVHD. PMID: 18814951

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Subcellular Location Mitochondrion, Membrane, Multi-pass membrane protein
Protein Families IFI6/IFI27 family
Database Links

HGNC: 5397

OMIM: 600009

KEGG: hsa:3429

STRING: 9606.ENSP00000298902

UniGene: Hs.532634

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