Human Interferon-induced 35 kDa protein(IFI35) ELISA kit

Instructions
Code CSB-EL011013HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interferon-induced protein 35
Alternative Names FLJ21753 ELISA Kit; IFI 35 ELISA Kit; Ifi-35 ELISA Kit; Ifi35 ELISA Kit; IFP 35 ELISA Kit; IFP35 ELISA Kit; IN35 ELISA Kit; IN35_HUMAN ELISA Kit; Interferon induced 35 kDa protein ELISA Kit; Interferon-induced 35 kDa protein ELISA Kit; OTTHUMP00000236626 ELISA Kit; OTTHUMP00000236627 ELISA Kit
Abbreviation IFI35
Uniprot No. P80217
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IFI35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 96-102
1:2 Average % 95
Range % 89-100
1:4 Average % 94
Range % 90-98
1:8 Average % 88
Range % 86-95
Recovery
The recovery of human IFI35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 99-108
EDTA plasma (n=4) 93 90-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.768 2.848 2.808 2.715
800 1.622 1.709 1.666 1.573
400 1.034 1.115 1.075 0.982
200 0.669 0.655 0.662 0.569
100 0.323 0.346 0.335 0.242
50 0.227 0.222 0.225 0.132
25 0.144 0.134 0.139 0.046
0 0.096 0.090 0.093  
.
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Not yet known.
Gene References into Functions
  1. IFI35 enhances proliferation of mesangial cells and is regulated by MBD2 in lupus nephritis. PMID: 28064541
  2. Damage-associated molecular patterns (DAMP) are important mediators of innate immunity. Here the authors show that N-myc and STAT interactor (NMI) and interferon-induced protein 35 (IFP35) act as DAMPs to promote inflammation by activating macrophages via the Toll-like receptor 4 and NF-kappaB pathways. PMID: 29038465
  3. Study found that TLR3 signaling induces the expression of IFI35, and IFI35 negatively regulates IFN-beta-P-STAT1-RIG-I-CXCL10/CCL5 axis in U373MG cells. This suggests that IFI35 expressed in astrocytes may play an important role in regulating the innate immune system in astrocytoma cells. PMID: 28109979
  4. Activation of TLR3 by poly IC induces the IFI35 expression in Mesangial Cells. Regional expression of IFI35 and its dysregulation may be involved in the pathogenesis of glomerular inflammation in CKD. PMID: 27639618
  5. Trim21 regulates Nmi-IFI35 complex-mediated inhibition of innate antiviral response PMID: 26342464
  6. Knockdown of IFP35 expression abolished pEGFP-N1-2C and pEGFP-N1-Nmi-induced activation of type I interferon promoters. PMID: 25085622
  7. IFI35 negatively regulates RIG-I antiviral signaling and supports vesicular stomatitis virus replication. PMID: 24371060
  8. IRF-1 also activates IFP35 expression in an IFN-gamma-inducible manner. PMID: 23226549
  9. Dissociation of the IFN-induced protein IFP35 from NMI is a newly defined specific cytoplasmic event occurring during apoptosis. PMID: 11911807
  10. The results provide a novel role of CKIP-1 in cytokine signaling response and the biochemical mechanism, by which two previously identified modulators IFP35 and Nmi are involved via interactions. PMID: 17197158
  11. IFP35 can interact with the bovine Tas (BTas) regulatory protein of bovine foamy virus; overexpression of IFP35 disturbs the ability of BTas to activate viral-gene transcription and inhibits viral replication. PMID: 18305040

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Subcellular Location Nucleus
Protein Families NMI family
Tissue Specificity In a wide range of cell types, including fibroblasts, macrophages, and epithelial cells.
Database Links

HGNC: 5399

OMIM: 600735

KEGG: hsa:3430

STRING: 9606.ENSP00000395590

UniGene: Hs.632258

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