Human Interferon regulatory factor 7(IRF7) ELISA kit

Instructions
Code CSB-EL011822HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interferon regulatory factor 7
Alternative Names IMD39 ELISA Kit; Interferon regulatory factor 7 ELISA Kit; Interferon regulatory factor 7H ELISA Kit; IRF 7 ELISA Kit; IRF 7A ELISA Kit; IRF 7H ELISA Kit; IRF-7 ELISA Kit; IRF7 ELISA Kit; IRF7_HUMAN ELISA Kit; IRF7A ELISA Kit; IRF7B ELISA Kit; IRF7C ELISA Kit; IRF7H ELISA Kit
Abbreviation IRF7
Uniprot No. Q92985
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.4 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IRF7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-94  
1:2 Average % 101  
Range % 96-104  
1:4 Average % 91  
Range % 84-95  
1:8 Average % 97  
Range % 92-103  
Recovery
The recovery of human IRF7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 87-97  
EDTA plasma (n=4) 94 88-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1500 2.291 2.166 2.229 2.026  
750 1.911 1.822 1.867 1.664  
375 1.447 1.456 1.452 1.249  
187.5 1.200 1.177 1.189 0.986  
94 0.811 0.801 0.806 0.603  
47 0.605 0.623 0.614 0.411  
23.5 0.354 0.333 0.344 0.141  
0 0.205 0.201 0.203    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
Gene References into Functions
  1. cFLIP appears to bind to IKKalpha to prevent IKKalpha from phosphorylating and activating IRF7. PMID: 29222334
  2. our results suggest that expression of IRF7 is one of the metastatic effectors of LMP1 signalling in Epstein-Barr virus-associated nasopharyngeal cancer PMID: 28712115
  3. Hypomethylation of the IRF7 promoter might play a role in systemic sclerosis pathogenesis, probably through promoting the IRF7 expression in PBMCs of patients with SSc. PMID: 28952189
  4. Results showed that INF-lambda serum concentration was increased in Alzheimer's disease (AD) and mild cognitive impairment (MCI) carrying the IFNL3 T allele compared to healthy controls (HC). Anti-HSV-1 Ab titers were higher in AD and MCI individuals carrying the IRF7 AA genotype compared to HC. IFNL3 rs12979860 and IRF7 rs6598008 polymorphisms may modulate immune responses against HSV-1 via their effect on the IFN-lam... PMID: 28984602
  5. IRF7 plays a role in reducing bone metastasis of prostate cancer by IFN-beta-mediated NK activity. PMID: 27733217
  6. The MYC is shown to be recruited to the IRF7 promoter region through interaction with nuclear receptor corepressor 2/histone deacetylase 3 for its repression. PMID: 27630164
  7. S100A9 knockdown almost completely abrogated the effects of IRF7 deletion on granulocytic myeloid-derived suppressor cells (G-MDSC) development and tumor metastasis. IRF7 represents a novel regulator for G-MDSC development in cancer and may have predictive value for tumor progression. PMID: 28092673
  8. the transcription factor NFATC3 binds to IRF7 and functions synergistically to enhance IRF7-mediated IFN expression in Plasmacytoid dendritic cells. PMID: 27697837
  9. We show that IRF-7 siRNA knockdown enhanced LPS-induced IL-10 production in human monocyte-derived macrophages, and USP-18 overexpression attenuated LPS-induced production of IL-10 in RAW264.7 cells. Quantitative PCR confirmed upregulation of USP18, USP41, IL10, and IRF7. An independent cohort confirmed LPS induction of USP41 and IL10 genes PMID: 27434537
  10. KSHV-encoded viral IRF4 interacts with the host IRF7 and inhibits interferon-alpha production. PMID: 28342865
  11. The adaptor molecule RAIDD coordinates IKKepsilon and IRF7 interaction to ensure efficient expression of type I interferon. PMID: 27606466
  12. Bcl6, by interacting with the co-factors NcoR2 and HDAC3, plays a pivotal role in controlling IRF7 induction and antiviral signaling priming. PMID: 26728228
  13. Human IRF7 was shown to be essential for interferon type I-dependent protective immunity against primary influenza. (Review) PMID: 26761402
  14. The IRF7 GG genotype associate with Cognitive Decline and Dementia. PMID: 25835418
  15. IRF7 cleavage by the 3C protease of enterovirus D68 abrogated its capacity to activate type I interferon expression and limit virus replication. PMID: 26608321
  16. Interferon regulatory factor 7 (IRF7) is a direct target of miR-762 and overexpression of miR-762 reduced expression of IRF7. PMID: 26597380
  17. Data indicate that the type-I interferon master regulator gene interferon regulatory factor 7 (IRF7) is only hypomethylated in lupus patients with renal involvement. PMID: 26005050
  18. our results indicate that IRF7 promotes glioma cell invasion and both chemoresistance and radioresistance through AGO2 inhibition PMID: 25680411
  19. The interaction between AIP and IRF7 is enhanced upon virus infection, and AIP potently inhibits IRF7-induced type I IFN (IFN-alpha/beta) production. PMID: 25911105
  20. Authors found that knockdown of IRF7 leads to growth inhibition of Epstein-Barr virus-transformed cells, and restoration of IRF7 by exogenous plasmid correlates with growth recovery of the viral transformed cells. PMID: 25300801
  21. In TSC2-deficient angiomyolipoma patient cells, IRF7 is a pivotal factor in the Rheb/mTOR pathway. PMID: 25476905
  22. These findings suggest that IRF7-dependent amplification of type I and III IFNs is required for protection against primary infection by influenza virus in humans. PMID: 25814066
  23. Authors conclude that paramyxoviruses trigger the DNA damage response, a pathway required for MSK1 activation of phospho Ser 276 RelA formation to trigger the IRF7-RIG-I amplification loop necessary for mucosal interferon production. PMID: 25520509
  24. phosphorylation-mediated IRF7 transactivation is controlled by a tripod-helix structure. PMID: 25225665
  25. Data indicate that knockdown of IRF-7 expression almost completely diminished the enhancing effect of TLR9 signaling on Foxp3 expression, suggesting that IRF-7 is a downstream molecule of TLR9 signaling. PMID: 23490285
  26. In over 800 breast cancer patients, high expression of Irf7-regulated genes in primary tumors was associated with prolonged bone metastasis-free survival. PMID: 22820642
  27. Tat interaction with the 2 MAPKK and IRF7 promoters in HIV-1-infected cells and the resulting persistent activation of interferon-stimulated genes, which include inflammatory cytokines and chemokines, can contribute to the increased immune activation. PMID: 23535064
  28. In the present study, the authors report that enterovirus 71 downregulates IRF7 through the 3C protein, which inhibits the function of IRF7. PMID: 23175366
  29. The major allele of a nonsynonymous polymorphism, rs1131665 (412Q) in IRF7, confers elevated activation of IRF-7 and predisposes to the development of systemic lupus erythematosus in multiple ethnic groups. PMID: 21360504
  30. None of the IRF7 polymorphisms was associated with systemic lupus erythematosus. PMID: 22433914
  31. phosphorylation of IRF7 on Ser477 and Ser479 by IKKepsilon or TBK1 is inhibited by KSHV ORF45 PMID: 22787218
  32. discuss the association of IRF7 and SLE based on recent understandings to render more information about the mechanisms of IRF7 might perform in PMID: 22455868
  33. Reconstruction of the wiring diagram of the modules revealed the presence of hyperconnected hub nodes, most notably interferon regulatory factor 7, which was identified as a major hub linking interferon-mediated antiviral responses. PMID: 22112518
  34. IRF7 single nucleotide polymorphism rs1061501 TT genotype and T allele are enriched in Taiwanese patients with systemic lupus erythematosus (SLE) and seem to be associated with an increased risk of developing SLE. PMID: 21632682
  35. Vesicular stomatitis Indiana virus-activated IRF7 upregulates expression of the BST2 gene independently of interferon signaling. PMID: 22301143
  36. TRAF6 can regulate HIV-1 production and expression of IRF7 promotes HIV-1 replication. PMID: 22140520
  37. IRF7 region is an anticentromere autoantibody propensity locus in systemic sclerosis PMID: 21926187
  38. Vaccinia virus protein C6 inhibits the activation of IRF7 by TBK1- and IKKepsilon-dependent pathways. PMID: 21931555
  39. The ubiquitin E3 ligase activity of tripartite motif-containing (TRIM)28 protein is specific to IRF7. PMID: 21940674
  40. Cigarette smoke suppresses key plasmacytoid dendritic cells functions upon viral infection by a mechanism that involves downregulation of TLR7 expression and decreased activation of IRF-7. PMID: 21435390
  41. Study did not find signi fi cant relationships between rs4963128 or rs2246614 of IRF7/KIAA1542 and the risk of systemic lupus erythematosus, differing from a previous study of European women PMID: 21167895
  42. analysis of IFN-stimulated response elements (ISREs) that bind to both the IFN-stimulated gene factor 3 (ISGF3) as well as to IFN response factor 7 (IRF7) PMID: 20943654
  43. ORF45 may maintain the IRF-7 molecule in the closed form and prevent it from being activated in response to viral infection PMID: 20980251
  44. These findings demonstrate that the lysine residues of IRF-7 play important roles in mediating IFN synthesis and modulating viral lytic replication. PMID: 20844090
  45. IRF-7-induced HPV8 transcription in primary keterinocytes. PMID: 20980500
  46. interferon regulatory factor 7C has dual roles in Epstein-Barr virus-mediated lymphocyte transformation PMID: 20209099
  47. HCV infection enhances STAT1 expression but impairs nuclear translocation of IRF-7 and its downstream molecules. These impairments in the IFN-alpha signaling pathway may, in part, be responsible for establishment of chronic HCV infection. PMID: 20810735
  48. Results suggest that Ro52-mediated ubiquitination promotes the degradation of IRF7 following TLR7 and TLR9 stimulation. PMID: 20668674
  49. EBV infection correlated with a blockage in the activation of JAK/STAT pathway members and affected the level of phosphorylated IFN regulatory factor 7 (IRF7). PMID: 20689596
  50. Smokers after infection with influenza is associated with reduced expression of IRF7 in nasal epithelial cells. PMID: 19880818
  51. In contrast, although expression of IRF-7 was higher in Gr II than in the other groups, there was no statistically significant difference (P > 0.05). PMID: 19858727
  52. A20 negatively regulates LMP1-stimulated IRF7 ubiquitination and activity in EBV latency. PMID: 20392859
  53. IRF7 is activated upon Chlamydia pneumoniae infection and is crucial for type I interferon-beta expression PMID: 20154210
  54. IRF7/PHRF1 variants in combination with systemic lupus erythematosus-associated autoantibodies result in higher serum levels of IFNalpha PMID: 20112359
  55. The structure and function of IRF7 are reviewed PMID: 11846980
  56. Stimulation of IRF-7 gene expression by tumor necrosis factor alpha: requirement for NFkappa B transcription factor and gene accessibility PMID: 11877397
  57. acetylation of lysine 92 negatively modulates IRF7 DNA binding PMID: 12374802
  58. Interferon regulatory factor-7 synergizes with other transcription factors through multiple interactions with p300/CBP coactivators. PMID: 12604599
  59. Interferon regulatory factor 7 regulates expression of Epstein-Barr virus latent membrane protein 1: a regulatory circuit. PMID: 12915551
  60. herpes simplex virus ICP0 blocks interferon regulatory factor IRF7-mediated activation of interferon-stimulated genes; the RING finger domain of ICP0 is essential for this activity PMID: 14747533
  61. LPS & HSV upregulate IRF-7 in plasmacytoid dendritic cells. This depends on NF-kappa B activation. Nuclear translocation of IRF-7 occurs rapidly in response to HSV. Activation of IRF-7 contributes to IFN-alpha production in virus-stimulated PDC. PMID: 15265881
  62. TLR-mediated IFN-alpha induction requires the formation of a complex consisting of MyD88, TRAF6 and IRF7 as well as TRAF6-dependent ubiquitination. PMID: 15361868
  63. there are two distinct mechanisms for the activation of the IRF7 promoter, by IFN and by virus infection PMID: 15664995
  64. LPS-induced B7.1 transcription in human monocytic cells may be regulated by JNK-mediated activation of the IRF-7 transcription factor. PMID: 16237059
  65. IRF7 and LMP1 interact with each other, and this may relate to the mechanism whereby LMP1 exerts functional effects in B-lymphocytes PMID: 16778376
  66. We show that BRCA1, signal transducer and activator of transcription (STAT)-1, and STAT2 are all required for the induction of IRF-7 following stimulation with IFN-gamma. PMID: 17374731
  67. expression patterns of IRF3 & IRF7 in normal lymph nodes, reactive hyperplastic lymph nodes & pediatric lymphomas; the number of IRF7-positive cells was found to be elevated in the reactive hyperplastic lymph nodes and pediatric lymphoma PMID: 17393359
  68. In addition to its defined role in type I interferon stimulation, IRF-7 plays a key role in modulating adaptive immune responses by inducing low molecular mass polypeptide-2 (LMP-2) expression, either directly or through induction of IRF-1. PMID: 17404045
  69. Results report a novel immune evasion mechanism of KSHV vIRF3 to block cellular IRF7-mediated innate immunity in response to viral infection. PMID: 17522209
  70. PI3K selectively controls type I IFN production by regulating IRF-7 nuclear translocation in human pDCs PMID: 18227218
  71. Our analyses showed a prominent effect of the IRF-7 nonsynonymous SNPs on the risk of developing cirrhosis. PMID: 18397234
  72. TRAF6 and its E3 ligase activity are required for LMP1-stimulated IRF7 ubiquitination. PMID: 18710948
  73. This screening demonstrates that LF2 specifically interacts with the central inhibitory association domain of IRF7, and this interaction leads to inhibition of the dimerization of IRF7, which suppresses IFN-alpha production and IFN-mediated immunity. PMID: 18987133
  74. efficient IRF7 activation required association with LMP1 CTAR2 in proximity to LMP1 CTAR2 mediated kinase activation sites PMID: 19017798

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Involvement in disease Immunodeficiency 39 (IMD39)
Subcellular Location Nucleus, Cytoplasm
Protein Families IRF family
Tissue Specificity Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
Database Links

HGNC: 6122

OMIM: 605047

KEGG: hsa:3665

STRING: 9606.ENSP00000329411

UniGene: Hs.166120

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