Human Interleukin-1 family member 10(IL1F10) ELISA kit

Instructions
Code CSB-EL011615HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name interleukin 1 family, member 10 (theta)
Alternative Names FIL1-theta, FKSG75, IL-1HY2, IL1-theta, MGC119831, MGC119832, MGC119833, IL-1F10 (canonical form IL-1F10a)|interleukin 1 family, member 10|interleukin-1 HY2|interleukin-1 receptor antagonist FKSG75|
Abbreviation IL1F10
Uniprot No. Q8WWZ1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-104
1:2Average %84
Range %80-88
1:4Average %105
Range %100-113
1:8Average %95
Range %90-100
Recovery
The recovery of human IL1F10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8880-92
EDTA plasma (n=4)9490-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
20002.070 2.217 2.144 2.044
10001.393 1.321 1.357 1.257
5000.756 0.809 0.783 0.683
2500.421 0.445 0.433 0.333
1250.267 0.254 0.261 0.161
62.50.196 0.183 0.190 0.090
31.250.151 0.154 0.153 0.053
00.103 0.096 0.100
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
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Target Data

Function Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2.
Subcellular Location Secreted
Protein Families IL-1 family
Tissue Specificity Expressed in fetal skin, spleen and tonsil. Expressed mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil.
Database Links

HGNC: 15552

OMIM: 615296

KEGG: hsa:84639

STRING: 9606.ENSP00000341794

UniGene: Hs.306974

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