Human Interleukin 12,IL-12/P70 ELISA Kit

This ELISA kit provides quantitative measurement of human IL-12/p70, the bioactive heterodimer (p35/p40) critical for Th1 polarization and IFN-γ induction, in serum, plasma, and cell culture supernatants. Because IL-12/p70 circulates at very low concentrations and is often the rate-limiting signal in Th1 commitment studies, precise detection at the picogram level is essential for meaningful data.

Detection Range: The 3.9–250 pg/ml range spans both the low baseline levels of IL-12/p70 found in healthy human serum (typically <5–20 pg/ml) and the elevated concentrations observed during active infection or following dendritic cell stimulation in vitro, minimizing the need for sample dilution or concentration in most experimental contexts.

Sensitivity: At 0.975 pg/ml, this kit detects IL-12/p70 in unstimulated or weakly stimulated conditions—critical for capturing early dendritic cell activation kinetics or distinguishing subtle differences in monocyte-derived IL-12 production between patient cohorts.

Sample Compatibility: Validation across serum, plasma, and cell culture supernatants enables direct comparison between ex vivo patient cytokine levels and in vitro stimulation assays using PBMCs or monocyte-derived dendritic cells within a single study.

Assay Efficiency: The 1–5 hour protocol requiring only 50–100 µl accommodates limited clinical specimens and small-scale primary cell cultures where IL-12/p70 output per well is constrained by low cell yields.

This kit supports research in sepsis immunopathology, vaccine adjuvant development, and Th1-mediated autoimmune disease profiling.