Mouse Interleukin 12,IL-12/P70 ELISA KIT

This ELISA kit quantitatively measures mouse IL-12/p70, the bioactive heterodimer of p35 and p40 subunits, in immunological research where distinguishing the intact cytokine from free subunits is critical. Because IL-12/p70 is produced transiently by activated dendritic cells and macrophages and circulates at low concentrations even during active Th1 responses, accurate detection demands both sufficient sensitivity and a well-calibrated working range.

Detection Range: The 7.8–500 pg/mL range spans the concentrations typically observed in mouse serum following infectious challenge or LPS stimulation, where IL-12/p70 levels often peak between 50 and 300 pg/mL. Most in vivo samples can be measured neat or with minimal dilution, simplifying assay planning.

Sensitivity: At 1.95 pg/mL, the kit detects IL-12/p70 in unstimulated or baseline samples where the heterodimer is near-absent, enabling kinetic studies that capture the earliest phases of innate immune activation.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates (50–100 µL) allows researchers to correlate systemic IL-12/p70 levels with local production in spleen, lymph node, or tumor microenvironment within a single experimental framework.

Assay Efficiency: The 1–5 hour protocol and low sample volume accommodate studies using small bleed volumes from serial sampling in mouse infection or vaccination models.

This kit supports research in intracellular infection, tumor immunosurveillance, and adjuvant-driven Th1 polarization.