| Code | CSB-E04600m-IS | 
| Size | 96T,5×96T,10×96T | 
| Price | Request a Quote | 
| Trial Size | 24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below. | 
| Have Questions? | Leave a Message or Start an on-line Chat | 
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| 
 | Sample | Serum(n=4) | ||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-95 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 91-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-99 | |||||
| The recovery of mouse IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 83-98 | ||||
| EDTA plasma (n=4) | 99 | 92-107 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| 
 | |||||||
| pg/ml | OD1 | OD2 | Average | Corrected | |||
| 500 | 2.862 | 2.734 | 2.798 | 2.603 | |||
| 250 | 2.052 | 1.967 | 2.010 | 1.815 | |||
| 125 | 1.366 | 1.358 | 1.362 | 1.167 | |||
| 62.5 | 0.885 | 0.860 | 0.873 | 0.678 | |||
| 31.2 | 0.551 | 0.541 | 0.546 | 0.351 | |||
| 15.6 | 0.400 | 0.383 | 0.392 | 0.196 | |||
| 7.8 | 0.303 | 0.294 | 0.298 | 0.103 | |||
| 0 | 0.198 | 0.193 | 0.195 | 
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