Mini Sample Mouse Interleukin 12,IL-12/P70 ELISA Kit

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Mini Sample Mouse Interleukin 12,IL-12/P70 ELISA Kit

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Protocol

Code	CSB-E04600m-MS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Target Name

Interleukin 12,IL-12/P70

Abbreviation

IL-12/P70

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

23.5pg/ml-1500pg/ml

Sensitivity

11.75pg/ml

Assay Time

3.5h

Sample Loading Volume

25ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	101
	Range %	93-106
1:2	Average %	88
	Range %	81-95
1:4	Average %	90
	Range %	82-95
1:8	Average %	107
	Range %	99-113

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	3.465	3.604	3.535	3.466
750	2.463	2.322	2.393	2.324
375	1.574	1.590	1.582	1.513
187.5	0.858	0.858	0.858	0.789
94	0.527	0.533	0.530	0.461
47	0.312	0.326	0.319	0.250
23.5	0.211	0.212	0.211	0.142
0	0.068	0.070	0.069

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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