Monkey Interleukin 12,IL-12/P70 ELISA Kit

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Monkey Interleukin 12,IL-12/P70 ELISA Kit

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Protocol

Code	CSB-E08904Mk
Size	96T,5×96T,10×96T
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Target Name

interleukin 12

Alternative Names

IL-12 elisa kit; P70 elisa kit

Abbreviation

IL-12/P70

Species

Monkey

Sample Types

serum, plasma

Detection Range

62.5 pg/mL-4000 pg/mL.

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of monkey IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	101
	Range %	96-108
1:2	Average %	96
	Range %	91-104
1:4	Average %	90
	Range %	84-98
1:8	Average %	86
	Range %	81-93

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
4000	2.651	2.571	2.611	2.516
2000	2.023	2.099	2.061	1.966
1000	1.421	1.462	1.442	1.347
500	0.905	0.898	0.902	0.807
250	0.574	0.541	0.558	0.463
125	0.396	0.410	0.403	0.308
62.5	0.254	0.246	0.250	0.155
0	0.093	0.097	0.095

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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