Human Interleukin 23,IL-23 ELISA Kit

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Human Interleukin 23,IL-23 ELISA Kit

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Protocol

Code	CSB-E08461h-IS
Size	96T,5×96T,10×96T
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Product Details
Customer Reviews and Q&A

Target Name

Interleukin 23,IL-23

Abbreviation

IL23

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

6.25pg/ml-400pg/ml

Sensitivity

3.125pg/ml

Assay Time

3.5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-96
1:2	Average %	94
	Range %	87-100
1:4	Average %	101
	Range %	96-107
1:8	Average %	97
	Range %	92-103

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	3.289	3.454	3.372	3.298
200	2.246	2.206	2.226	2.152
100	1.200	1.218	1.209	1.135
50	0.638	0.621	0.629	0.556
25	0.375	0.366	0.371	0.297
12.5	0.224	0.217	0.221	0.147
6.25	0.155	0.146	0.150	0.077
0	0.074	0.074	0.074

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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