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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of human IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||93||89-99|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
The human IL-23 ELISA Kit is engineered for accurate measurement of human IL-23 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the IL-23 content in the sample. The color intensity is positively correlated with IL-23 content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
IL-23 is a heterodimeric cytokine consisting of an IL-12p40 subunit and an IL-23-specific p19 subunit. It is secreted by activated dendritic cells and macrophages and can specifically amplify and stabilize the proliferation of IL-17-secreting Th17 cells. The IL-23/Th17 pathway has been involved in the development of many autoimmune diseases, such as rheumatoid arthritis (RA), systemic lupus erythematosus, and psoriatic arthritis (PsA). IL-23 signals through the IL-23R in complex with IL-12Rβ1, inducing the generation of secreted mediators such as IL-17 and IL-22, in pro-inflammatory, IL-17-secreting TCRγδ (Tγδ17), and in Th17 cells. These pro-inflammatory mediators facilitate the rapid recruitment and activation of granulocytes and macrophages that eventually result in the development of clinical symptoms.
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I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??
I am interested in this kit. I would like to know which subunit of IL-23 can be detected by the kit.
Please provide a detailed information about kit’s components:
a. Capture antibody or capture antigen.
b. Detection antibody (if applicable).
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
Thank you for your assistance.
Is the 293 protein you are referring the IL-23 p19 subunit?
Because you just mention the immunogen is 293 cell-derived recombinant protein. Please clarify, what is the 293 cell-derived protein specifically? For example, is it the IL-23 p19 subunit?
It is good to know, if the antibodies bind to the p40 unit or the p19 subunit?
Looking forward to receiving your feedback.