Rat Interleukin 23,IL-23 ELISA Kit

Rat Interleukin 23,IL-23 ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Rat Interleukin 23,IL-23 ELISA Kit

Name: Rat Interleukin 23,IL-23 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08462r

Citations (12) Protocol MSDS

Code	CSB-E08462r
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (12)
Related Products
Customer Reviews and Q&A

Target Name

Interleukin 23,IL-23

Alternative Names

N/A

Abbreviation

IL-23

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-94
1:2	Average %	104
	Range %	100-107
1:4	Average %	92
	Range %	87-95

Recovery

The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-95
EDTA plasma (n=4)	98	94-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.147	2.112	2.130	1.998
100	1.851	1.776	1.814	1.682
50	1.308	1.288	1.298	1.166
25	0.903	0.898	0.901	0.769
12.5	0.621	0.614	0.618	0.486
6.25	0.375	0.359	0.367	0.235
3.12	0.236	0.224	0.230	0.098
0	0.134	0.130	0.132

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat IL-23 ELISA Kit was designed for the quantitative measurement of Rat IL-23 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

Regulation of Dendritic Cell Function by Ermiaosan via the EP4-cAMP-CREB Signaling Pathway JM Ding, L Min, W Jin, SM Cheng, XW Meng,arXiv,2025
Imatinib mitigates experimentally-induced ulcerative colitis: Possible contribution of NF-kB/JAK2/STAT3/COX2 signaling pathway M Shalaby,Life sciences,2023
JAK2 inhibitor ameliorates the progression of experimental autoimmune myasthenia gravis and balances Th17/Treg cells via regulating the JAK2/ STAT3-AKT/mTOR signaling pathway Y Lu,International immunopharmacology,2023
Egg white hydrolysate from simulated gastrointestinal digestion alleviates the inflammation and improves the nutritional status in TNBS-induced Crohn’s disease rats T Wu,Journal of Functional Foods,2022
Mesenchymal stem cells moderate experimental autoimmune uveitis by dynamic regulating Th17 and Breg cells response Yajun Mu,Journal of Tissue Engineering and Regenerative Medicine,2021
The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells J Ding,Evidence-Based Complementary and Alternative Medicine,2020
Mahuang Fuzi Xixin Decoction Ameliorates Allergic Rhinitis in Rats by Regulating the Gut Microbiota and Th17/Treg Balance X Liang,Journal of Immunology Research,2020
Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats YuLong.et al,Biomedicine & Pharmacotherapy,2018
Matrine improves cognitive impairment and modulates the balance of Th17/Treg cytokines in a rat model of AB1-42-induced Alzheimer's disease Zhang Y.et al,Cent Eur J Immunol.,2016
Synthetic p55 tandem DNA vaccine against Pneumocystis carinii in rats. Fan H.et al,Microbiol Immunol.,2016
Mast cells mediate Pseudomonas aeruginosa lipopolysaccharide-induced lung inflammation in rat B. V. Lecirc,Tumor Biology,2012
Modulation of Pseudomonas aeruginosa lipopolysaccharide-induced lung inflammation by chronic iron overload in rat Baacute Vuong Lê,International Journal of Rheumatic Diseases,2012

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■ Q&A

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit

If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.