Name: Mouse Interleukin 23,IL-23 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08463m

Product Details

Description

This mouse IL-23 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse IL-23 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the IL-23 levels in the sample and is measured at 450 nm through a microplate reader.

IL-23 is mainly produced by macrophages and dendritic cells, in response to exogenous or endogenous signals, and drives the differentiation and activation of Th17 cells with subsequent production of IL-17A, IL-17F, IL-6, IL-22, and TNF-α. IL-23 signals through its heterodimeric receptor complex consisting of the two subunits IL-12Rβ1 and IL-23R, activating P38/MAPK, PI3K-Akt, or the NF-кB pathways. Although IL-23 plays a pivotal role in the protective immune response to bacterial and fungal infections, its dysregulation has been shown to exacerbate chronic immune-mediated inflammation. IL-23 is involved in the pathogenesis of Crohn’s Disease, Psoriasis, and Psoriatic Arthritis.

Target Name

Interleukin 23,IL-23

Alternative Names

N/A

Abbreviation

IL-23

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.56 pg/mL-100 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-92
1:2	Average %	95
	Range %	89-100
1:4	Average %	93
	Range %	84-97
1:8	Average %	106
	Range %	97-110

Recovery

The recovery of mouse IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	94-108
EDTA plasma (n=4)	101	94-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.868	2.689	2.779	2.654
250	1.519	1.586	1.553	1.428
125	0.746	0.700	0.723	0.598
62.5	0.434	0.456	0.445	0.320
31.2	0.314	0.301	0.308	0.183
15.6	0.207	0.202	0.205	0.080
7.8	0.153	0.159	0.156	0.031
0	0.127	0.123	0.125

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Customer Reviews and Q&A

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■ Q&A

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit

If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!

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Code	CSB-E08463m
Size	96T,5×96T,10×96T
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Mouse Interleukin 23,IL-23 ELISA Kit

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