Interleukin 25 is a cytokine that belongs to the IL-17 family and plays a key role in type 2 immune responses. Epithelial cells primarily produce IL-25, particularly in barrier tissues such as the lungs and intestines, where it functions as an important mediator in allergic inflammation and host defense against parasitic infections. This cytokine activates innate lymphoid cells type 2 and promotes the production of type 2 cytokines, making it a significant target for research in asthma, allergic diseases, and immune regulation studies.
The Human Interleukin 25 ELISA kit (CSB-E11715h) uses a quantitative sandwich measurement principle for detecting IL-25 in human samples. This assay accommodates serum, cell culture supernates, urine, and tissue homogenates with a detection range of 62.5 pg/mL to 4000 pg/mL and sensitivity of 15.6 pg/mL. The protocol requires 50-100 μL sample volume, operates with a 1-5 hour assay time, and uses detection at 450 nm wavelength.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in clinical research studies to measure interleukin 25 levels in human biological samples as part of comprehensive cytokine profiling work. The applications span allergy research and respiratory disease studies, where researchers have quantified this biomarker alongside other inflammatory mediators to better understand disease mechanisms and immune responses.
• Allergy research: Measurement of interleukin 25 concentrations in serum samples from patients with food allergies, particularly in studies comparing allergic patients to healthy control groups to examine immune system responses
• Respiratory disease studies: Measurement of interleukin 25 levels in bronchoalveolar lavage fluid and nasal lavage samples to evaluate inflammatory processes in the respiratory tract
• Cytokine profiling: Integration into multi-analyte panels for comprehensive analysis of inflammatory mediators, allowing researchers to study complex immune responses and cytokine networks in various disease contexts
• Biomarker research: Quantification of interleukin 25 as part of broader studies into inflammatory biomarkers associated with allergic and respiratory conditions
