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| Code | CSB-E11715h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-103 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-106 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| The recovery of human IL-25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-89 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human IL-25 ELISA kit is suitable for the quantitative determination of human IL-25 in different sample types, including serum, cell culturesupernates, urine, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.
IL-25, generated by epithelial cells along with numerous innate immune cells, plays an important role in increasing Th2 cytokine synthesis. Once secreted, IL-25 can bind to the receptors, IL-17RA and IL-17RB, and activate the NF-κB pathway where it plays the role of a Th2 cell-driving cytokine. It participates in type 2 responses by promoting the production of Th2 cytokines and eosinophil infiltration. IL-25 is a major regulator that mediates innate and adaptive through the activation of ILC2s and Th2 cells to stimulate sustained Th2-skewed mucosal inflammation. Additionally, IL-25 has anti-inflammatory effects in autoimmune and inflammatory diseases via the downregulation of Th1 and Th17 cell responses.
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