Human Interleukin-36 beta(IL1F8) ELISA kit

Instructions
Code CSB-EL011619HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 1 family, member 8 (eta)
Alternative Names 2310043N20Rik ELISA Kit; Family of interleukin 1 eta ELISA Kit; FIL1 (ETA) ELISA Kit; FIL1 ELISA Kit; FIL1 eta ELISA Kit; FIL1H ELISA Kit; FILI-(ETA) ELISA Kit; IL 1F8 (FIL1 eta ELISA Kit; IL 1F8 ELISA Kit; IL 1H2 ELISA Kit; IL-1 eta ELISA Kit; IL-1F8 ELISA Kit; IL-1H2 ELISA Kit; IL1 ETA ELISA Kit; IL1F8 (Canonical product IL 1F8a) ELISA Kit; IL1F8 ELISA Kit; IL1F8_HUMAN ELISA Kit; IL1H2 ELISA Kit; IL36 beta ELISA Kit; IL36B ELISA Kit; Interleukin 1 family, member 8 (eta) ELISA Kit; Interleukin 1 family, member 8 ELISA Kit; Interleukin 1 homolog 2 ELISA Kit; Interleukin 1 Superfamily e ELISA Kit; Interleukin 36 beta ELISA Kit; Interleukin-1 eta ELISA Kit; Interleukin-1 family member 8 ELISA Kit; Interleukin-1 homolog 2 ELISA Kit; MGC126880 ELISA Kit; MGC126882 ELISA Kit; OTTMUSP00000012797 ELISA Kit; RP23-176J12.1 ELISA Kit
Abbreviation IL1F8
Uniprot No. Q9NZH7
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %84-92
1:2Average %96
Range %90-102
1:4Average %89
Range %85-94
1:8Average %88
Range %84-95
Recovery
The recovery of human IL1F8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8680-92
EDTA plasma (n=4)10096-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.537 2.598 2.568 2.404
5002.062 2.091 2.077 1.913
2501.399 1.368 1.384 1.220
1250.799 0.771 0.785 0.621
62.50.555 0.534 0.545 0.381
31.20.374 0.395 0.385 0.221
15.60.271 0.294 0.283 0.119
00.163 0.165 0.164  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6.
Gene References into Functions
  1. IL-36-mediated IL-6 and CXCL8 production in human lung fibroblasts and bronchial epithelial cells may be involved in pulmonary inflammation especially caused by bacterial or viral infections. PMID: 28869889
  2. With a focus on the skin as a target for microbial and viral invasion, the current knowledge of IL-36: IL-36alpha, IL-36beta and IL-36gamma, functions is reviewed. One physiological function of the IL-36smay be to counteract microbial immune evasion. [Review] PMID: 28811383
  3. IL36B expression is induced in keratinocytes by Toll-like receptor ligands suggesting a function in barrier immune defense. PMID: 22318382
  4. IL-1F8 functions as an inducer of antimicrobial peptide and matrix metalloproteinase expression by keratinocytes; IL-1F8 is overexpressed in transgenic psoriatic model mouse skin. PMID: 21242515
  5. IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells PMID: 14734551
  6. Joint and serum IL-1F8 protein levels did not correlate with inflammation, but they were high in samples from patients with rheumatoid arthritis PMID: 16646978

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Subcellular Location Secreted
Protein Families IL-1 family
Tissue Specificity Expression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in any hematopoietic cell lines. Not detected in adipose tissue. Expressed at higher levels in psoriatic plaques than in symptomless psoriatic skin or healthy
Database Links

HGNC: 15564

OMIM: 605508

KEGG: hsa:27177

STRING: 9606.ENSP00000259213

UniGene: Hs.278909

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