Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of >1.0x10⁵ IU/mg.

Target Names

IL36B

Uniprot No.

Q9NZH7

Research Area

Immunology

Alternative Names

2310043N20Rik; Family of interleukin 1 eta; FIL1 (ETA); FIL1; FIL1 eta; FIL1H; FILI-(ETA); IL 1F8 (FIL1 eta; IL 1F8; IL 1H2; IL-1 eta; IL-1F8; IL-1H2; IL1 ETA; IL1F8 (Canonical product IL 1F8a); IL1F8; IL1F8_HUMAN; IL1H2; IL36 beta; IL36B; Interleukin 1 family, member 8 (eta); Interleukin 1 family, member 8; Interleukin 1 homolog 2; Interleukin 1 Superfamily e; Interleukin 36 beta; Interleukin-1 eta; Interleukin-1 family member 8; Interleukin-1 homolog 2; MGC126880; MGC126882; OTTMUSP00000012797; RP23-176J12.1

Species

Homo sapiens (Human)

Source

E.Coli

Expression Region

5-157aa

Complete Sequence

REAAPKSYAI RDSRQMVWVL SGNSLIAAPL SRSIKPVTLH LIACRDTEFS DKEKGNMVYL GIKGKDLCLF CAEIQGKPTL QLKEKNIMDL YVEKKAQKPF LFFHNKEGST SVFQSVSYPG WFIATSTTSG QPIFLTKERG ITNNTNFYLD SVE

Mol. Weight

17.2 kDa

Protein Length

Partial of Isoform 2

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder

Buffer

0.2 μm filtered 2 x PBS, pH 7.4 ,lyophilized

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Datasheet & COA

Please contact us to get it.

Description

IL-36β (IL-1F8) signals through the IL-36 receptor to activate NF-κB and MAPK pathways, playing a central role in epithelial and immune cell inflammatory responses. This tag-free recombinant human IL-36β, spanning residues 5–157 of Isoform 2, faithfully represents the processed mature sequence and demonstrates strong signaling potency with an ED50 below 10 ng/mL in an IL-8 secretion assay using human preadipocytes, corresponding to a specific activity exceeding 1.0 × 10⁵ IU/mg. These activity data support use in cell-based functional assays measuring NF-κB or MAPK activation, downstream cytokine release studies, in vivo inflammation models, and as a quantitative reference standard in cytokine detection platforms such as ELISA. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell experiments, while purity greater than 97% by SDS-PAGE provides a suitable basis for antibody development workflows including immunization and positive control applications.

Target Background

Function

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6.

Gene References into Functions

IL-36-mediated IL-6 and CXCL8 production in human lung fibroblasts and bronchial epithelial cells may be involved in pulmonary inflammation especially caused by bacterial or viral infections. PMID: 28869889
With a focus on the skin as a target for microbial and viral invasion, the current knowledge of IL-36: IL-36alpha, IL-36beta and IL-36gamma, functions is reviewed. One physiological function of the IL-36smay be to counteract microbial immune evasion. [Review] PMID: 28811383
IL36B expression is induced in keratinocytes by Toll-like receptor ligands suggesting a function in barrier immune defense. PMID: 22318382
IL-1F8 functions as an inducer of antimicrobial peptide and matrix metalloproteinase expression by keratinocytes; IL-1F8 is overexpressed in transgenic psoriatic model mouse skin. PMID: 21242515
IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells PMID: 14734551
Joint and serum IL-1F8 protein levels did not correlate with inflammation, but they were high in samples from patients with rheumatoid arthritis PMID: 16646978

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Subcellular Location

Cytoplasm. Secreted.

Protein Families

IL-1 family

Tissue Specificity

Expression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in any hematopoietic cell lines. Not detected in adipose tissue. Expressed at higher levels in psoriatic plaques than in symptomless psoriatic skin or healthy

Database Links

HGNC: 15564

OMIM: 605508

KEGG: hsa:27177

STRING: 9606.ENSP00000259213

UniGene: Hs.278909

Code	CSB-AP002021HU
Abbreviation	Recombinant Human IL36B protein, partial (Active)
MSDS	MSDS Datasheet
Size	$354
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Recombinant Human Interleukin-36 beta (IL36B),Partial (Active)

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