This human IL-37 ELISA Kit is suitable for qualitatively determining human concentrations in multiple biological fluids, including human serum, plasma, and tissue homogenates in vitro. IL-37 plays an important role in innate and adaptive immunity and may be a useful molecule for effective cytokine therapy. During inflammation, IL-37 inhibits the expression of several pro-inflammatory cytokines to facilitate the expression of the anti-inflammatory ones by regulating macrophage polar. In addition to modulating innate and acquired immunity, IL-37 also improves aging-associated immunosenescence. Furthermore, IL-37 exhibits an inhibitory effect on tumor angiogenesis and metastasis and progression.
This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human IL-37 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human IL-37 antibody. Biotin-labeled IL-37 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the IL-37n in the human samples. IL-37 binds to the biotinylated anti-IL-37 human monoclonal antibody. And the biotin on the biotinylated anti-IL-37 human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of IL-37 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.