Target Name
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
Alternative Names
KAIN_HUMAN ELISA Kit; KAL ELISA Kit; Kallikrein binding protein ELISA Kit; Kallikrein inhibitor ELISA Kit; Kallistatin ELISA Kit; KBP ELISA Kit; KLST ELISA Kit; KST ELISA Kit; MGC108582 ELISA Kit; Peptidase inhibitor 4 ELISA Kit; PI-4 ELISA Kit; PI4 ELISA Kit; Protease inhibitor 4 (kallistatin) ELISA Kit; Protease inhibitor 4 ELISA Kit; Serine (or cysteine) proteinase inhibitor; clade A (alpha 1 antiproteinase; antitrypsin); member 4 ELISA Kit; Serpin A4 ELISA Kit; Serpin peptidase inhibitor clade A member 4 ELISA Kit; Serpin peptidase inhibitor; clade A (alpha 1 antiproteinase; antitrypsin); member 4 ELISA Kit; SERPINA4 ELISA Kit; Tissue kallistatin inhibitor ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5000 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:10000 |
Average % |
103 |
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| Range % |
98-108 |
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| 1:20000 |
Average % |
92 |
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| Range % |
90-98 |
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| 1:40000 |
Average % |
94 |
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| Range % |
92-96 |
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Recovery
| The recovery of human SERPINA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
93-104 |
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| EDTA plasma (n=4) |
94 |
89-97 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.432 |
2.565 |
2.499 |
2.337 |
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| 5 |
2.056 |
2.066 |
2.061 |
1.899 |
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| 2.5 |
1.442 |
1.457 |
1.450 |
1.288 |
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| 1.25 |
0.889 |
0.956 |
0.923 |
0.761 |
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| 0.625 |
0.535 |
0.544 |
0.540 |
0.378 |
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| 0.312 |
0.355 |
0.375 |
0.365 |
0.203 |
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| 0.156 |
0.268 |
0.274 |
0.271 |
0.109 |
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| 0 |
0.168 |
0.155 |
0.162 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Human SERPINA4 ELISA Kit was designed for the quantitative measurement of Human SERPINA4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
