Recombinant Human Kallistatin (SERPINA4)

Code CSB-YP021060HU
MSDS
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Source Yeast
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Code CSB-EP021060HU
MSDS
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Source E.coli
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Code CSB-EP021060HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021060HU
MSDS
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Source Baculovirus
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Code CSB-MP021060HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SERPINA4
Uniprot No.
Alternative Names
KAIN_HUMAN; KAL; Kallikrein binding protein; Kallikrein inhibitor; Kallistatin; KBP; KLST ; KST; MGC108582; Peptidase inhibitor 4; PI-4; PI4; Protease inhibitor 4 (kallistatin); Protease inhibitor 4; Serine (or cysteine) proteinase inhibitor; clade A (alpha 1 antiproteinase; antitrypsin); member 4; Serpin A4; Serpin peptidase inhibitor clade A member 4; Serpin peptidase inhibitor; clade A (alpha 1 antiproteinase; antitrypsin); member 4; SERPINA4; Tissue kallistatin inhibitor
Species
Homo sapiens (Human)
Expression Region
21-427
Target Protein Sequence
QLHVEHDGES CSNSSHQQIL ETGEGSPSLK IAPANADFAF RFYYLIASET PGKNIFFSPL SISAAYAMLS LGACSHSRSQ ILEGLGFNLT ELSESDVHRG FQHLLHTLNL PGHGLETRVG SALFLSHNLK FLAKFLNDTM AVYEAKLFHT NFYDTVGTIQ LINDHVKKET RGKIVDLVSE LKKDVLMVLV NYIYFKALWE KPFISSRTTP KDFYVDENTT VRVPMMLQDQ EHHWYLHDRY LPCSVLRMDY KGDATVFFIL PNQGKMREIE EVLTPEMLMR WNNLLRKRNF YKKLELHLPK FSISGSYVLD QILPRLGFTD LFSKWADLSG ITKQQKLEAS KSFHKATLDV DEAGTEAAAA TSFAIKFFSA QTNRHILRFN RPFLVVIFST STQSVLFLGK VVDPTKP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.
Gene References into Functions
  1. Kallistatin levels may provide useful information regarding cardiovascular risk in women with polycystic ovary syndrome PMID: 28294594
  2. Kallistatin functions as an endogenous lymphangiogenesis inhibitor and has an important part in the lymphatic metastasis of gastric cancer.Kallistatin was reduced in the cancer tissue and plasma of gastric cancer patients. PMID: 29243194
  3. SNPs rs2093266 in the SERPINA4 is associated with the development of severe acute kidney injury (KDIGO stage 2-3) in critically ill patients with septic shock PMID: 28270177
  4. oxidative stress induces down- or upregulation of kallistatin expression, depending on oxygen concentration, and kallistatin plays a novel role in mediating oxygen/exercise-induced HIF-1-eNOS-NO pathway. PMID: 29387292
  5. These findings reveal novel mechanisms of kallistatin in protection against senescence, aging, and cancer development by modulating miR-34a and miR-21 levels and inhibiting oxidative stress. PMID: 28744338
  6. These findings suggested that kallistatin may be a promising agent that could be used to suppress cancer metastasis by inhibiting both angiogenesis and lymphangiogenesis. PMID: 28440474
  7. generated a meta-marker model made up of SERPINA4 and PON1 that is useful as a differential diagnostic biomarker differentiating lung cancer from other lung diseases. PMID: 27168012
  8. The kallistatin's dual roles in angiogenesis, apoptosis and oxidative stress contribute to its beneficial effects in various diseases. PMID: 28742513
  9. Our results indicate that during severe sepsis and septic shock, a decrease in plasma concentrations of kallistatin reflects increased severity and poorer outcome of disease. PMID: 28542440
  10. data suggest that overexpression of kallistatin interferes with lymphopoiesis, ultimately impacting the level of circulating CD19(+) B lymphocytes. PMID: 28299632
  11. Kallistatin levels were correlated with some markers of systemic inflammation in the HIV population. PMID: 27326658
  12. Increased kallistatin levels in type 1 diabetes and its relation with carotid intima media thickness may reflect vascular dysfunction and suggest a link between micro- and macro-angiopathy. PMID: 26091968
  13. reveal novel mechanisms of kallistatin in inducing apoptosis and autophagy in breast cancer cells PMID: 26790955
  14. crystal structure of kallistatin 1.9 A resolution PMID: 26323298
  15. Kallistatin protects against renal ischemia-reperfusion injury by blocking oxidative stress and renal inflammation. PMID: 25654330
  16. Plasma kallistatin is a novel marker for severe community-acquired pneumonia prognosis. PMID: 23394256
  17. decreased SPTBN1 and kallistatin gene expression associated with decreased relapse-free survival in hepatocellular carcinoma PMID: 25307947
  18. these findings added the knowledge for us to understand the anti-angiogenic mechanism of kallistatin, which suggested that the rhKal could be worth as a candidate compound for further development for the purpose of anti-angiogenic therapies PMID: 24129914
  19. A novel role of kallistatin in preventing breast tumor growth and mobility by direct interaction with LRP6. PMID: 23666756
  20. As a potent antioxidant and anti-inflammation agent, kallistatin may also hold therapeutic promise in cardiometabolic disorders. PMID: 23190873
  21. Genotyping reveals two single nucleotide polymorphisms in the BCL2 gene and a single nucleotide polymorphism in the SERPINA4 gene associated with a decreased risk of developing acute kidney injury. PMID: 22710204
  22. Systemic administration of lentiviral vectors encoding kallistatin inhibited the growth of metastatic lung tumors and prolonged the survival of tumor-bearing mice. PMID: 20509975
  23. Delivery of human kallistatin cDNA to ischemic rat hindlimb and breast tumor xenografts showed that Kallistatin is a new inhibitor of angiogenesis and tumor growth. PMID: 12384424
  24. Results indicate that the heparin-binding domain, but not the reactive site loop of kallistatin, is essential for inhibiting vascular endothelial growth factor-induced angiogenesis. PMID: 12734113
  25. KBP reduces expression of VEGF and HIF-1alpha nuclear translocation PMID: 17714861
  26. Adeno-associated virus-mediated expression of KAL inhibits the growth of colon cancer by reducing angiogenesis and proliferation of tumor cells. PMID: 17729417

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Subcellular Location
Secreted.
Protein Families
Serpin family
Tissue Specificity
Expressed by the liver and secreted in plasma.
Database Links

HGNC: 8948

OMIM: 147935

KEGG: hsa:5267

STRING: 9606.ENSP00000298841

UniGene: Hs.719893

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