Human Ketohexokinase(KHK) ELISA kit

Code CSB-EL012157HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
ketohexokinase (fructokinase)
Alternative Names
EC 2.7.1.3 ELISA Kit; Fructokinase ELISA Kit; Hepatic fructokinase ELISA Kit; Ketohexokinase ELISA Kit; Ketohexokinase isoform a ELISA Kit; KHK ELISA Kit; KHK_HUMAN ELISA Kit
Abbreviation
KHK
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
18.75 mU/mL-1200 mU/mL
Sensitivity
4.68 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human KHK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %84-94
1:2Average %84
Range %80-88
1:4Average %95
Range %90-99
1:8Average %99
Range %93-103
Recovery
The recovery of human KHK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-102
EDTA plasma (n=4)9086-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
12002.547 2.461 2.504 2.352
6002.143 2.076 2.110 1.958
3001.403 1.449 1.426 1.274
1500.814 0.765 0.790 0.638
750.304 0.325 0.315 0.163
37.50.226 0.218 0.222 0.070
18.750.182 0.169 0.176 0.024
00.156 0.147 0.152  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human KHK ELISA Kit was designed for the quantitative measurement of Human KHK protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 mU/mL-1200 mU/mL and the sensitivity is 4.68 mU/mL .

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Target Background

Function
(From Uniprot)
Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
Gene References into Functions
  1. Data indicate metabolic enzymes NAD kinase and ketohexokinase as candidate metabolic gene targets, and the chromatin remodeling protein INO80C as a tumor suppressor in KRAS(MUT) colorectal tumor xenograft. PMID: 28954733
  2. Angelica archangelica, Garcinia mangostana, Petroselinum crispum, and Scutellaria baicalensis were the top four botanical candidiates identified with inhibitory activity against ketohexokinase-C. PMID: 27322374
  3. compared with normal hepatocytes, hepatocellular carcinoma (HCC) cells markedly reduce the rate of fructose metabolism and the level of reactive oxygen species, as a result of a c-Myc-dependent and heterogeneous nuclear ribonucleoprotein (hnRNP) H1- and H2-mediated switch from expression of the high-activity fructokinase (KHK)-C to the low-activity KHK-A isoform. PMID: 27088854
  4. myocardial hypoxia actuates fructose metabolism in human and mouse models of pathological cardiac hypertrophy through hypoxia-inducible factor 1alpha (HIF1alpha) activation of SF3B1 and SF3B1-mediated splice switching of KHK-A to KHK-C PMID: 26083752
  5. These studies identify fructokinase as a novel mediator of diabetic nephropathy and document a novel role for endogenous fructose production, or fructoneogenesis, in driving renal disease. PMID: 24876114
  6. This study determined if single nucleotide polymorphisms in genes involved in fructose transport,SLC2A2 and SLC2A5 and metabolism, etohexokinase affect inter-individual variability in metabolic phenotypes. PMID: 23341889
  7. In human hepatocytes uric acid up-regulates KHK expression thus leading to the amplification of the lipogenic effects of fructose. PMID: 23112875
  8. ketohexokinase-A serves an unknown physiologic function that remains intact in essential fructosuria. PMID: 12941785
  9. The expression of ketohexokinase is diminished in human clear cell type of renal cell carcinoma PMID: 16372272
  10. Ketohexokinase-dependent metabolism of fructose induces proinflammatory mediators in proximal tubular cells. PMID: 19158351
  11. The structure of the KHK-A ternary complex revealed an active site with fructose & the ATP analogue in positions ready for phosphorylation. The effects of the pathogenic mutations Gly40Arg & Ala43Thr have been modelled in the context of the KHK structure. PMID: 19237742

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Involvement in disease
Fructosuria (FRUCT)
Protein Families
Carbohydrate kinase PfkB family
Tissue Specificity
Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
Database Links

HGNC: 6315

OMIM: 229800

KEGG: hsa:3795

STRING: 9606.ENSP00000260598

UniGene: Hs.567297

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