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| Code | CSB-E13422h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of Human LN-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-106 | |||||
| The recovery of Human LN-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human LAMA5 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human LAMA5 in serum and plasma. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of LAMA5 bound in the initial step.
The LAMA5 gene codes for the laminin subunit α5, the most abundant laminin α subunit in the human brain. It forms a heterotrimer with subunit β1/β2 and γ1/γ3 and modulates neurodevelopmental processes, including epiblast polarization, neurite outgrowth, neuronal migration, synaptic stability, cell adhesion, differentiation, migration, and signaling transduction. LAMA5 plays a prominent role in the maintenance and function of the extracellular matrix (ECM) in mammalian tissues, which is crucial in developmental patterning, stem cell niches, cancer, and genetic diseases. It is necessary for normal embryonic development.
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