Human Layilin(LAYN) ELISA kit

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Human Layilin(LAYN) ELISA kit

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Protocol MSDS

Code	CSB-EL012772HU
Size	96T,5×96T,10×96T
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Target Background

Target Name

layilin

Alternative Names

Layilin ELISA Kit; LAYN ELISA Kit; LAYN_HUMAN ELISA Kit; UNQ208/PRO234 ELISA Kit

Abbreviation

LAYN

Uniprot No.

Q6UX15

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LAYN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	92
	Range %	89-96
1:4	Average %	104
	Range %	101-107
1:8	Average %	89
	Range %	85-93

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.321

2.341

2.331

2.159

500

1.929

1.902

1.916

1.744

250

1.395

1.376

1.386

1.214

125

0.875

0.841

0.858

0.686

62.5

0.574

0.592

0.583

0.411

31.25

0.396

0.387

0.392

0.220

15.6

0.287

0.291

0.289

0.117

0.171

0.173

0.172

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Receptor for hyaluronate.

Gene References into Functions

Renal biopsy samples from patients with glomerulonephritis showed high expression of LAYN in tubular epithelial cells. PMID: 26410531
results indicate that LAYN would be involved in the enhancement of inflammation and degradation of cartilage in joint diseases such as RA and OA PMID: 25150153
Hyaluronan and layilin mediate loss of airway epithelial barrier function induced by cigarette smoke by decreasing E-cadherin. PMID: 23048036

Subcellular Location

Membrane; Single-pass type I membrane protein.

Database Links

HGNC: 29471

KEGG: hsa:143903

STRING: 9606.ENSP00000364764

UniGene: Hs.503831

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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