Human Lipocalin-1(LCN1) ELISA kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

lipocalin 1 (tear prealbumin)

Alternative Names

LCN1 ELISA Kit; LCN1_HUMAN ELISA Kit; Lipocalin 1 (protein migrating faster than albumin, tear prealbumin) ELISA Kit; Lipocalin 1 like 2 ELISA Kit; Lipocalin-1 ELISA Kit; PMFA ELISA Kit; Tear lipocalin ELISA Kit; Tear prealbumin ELISA Kit; Tlc ELISA Kit; TP ELISA Kit; VEG protein ELISA Kit; VEGP ELISA Kit; Von Ebner gland protein ELISA Kit

Abbreviation

LCN1

Uniprot No.

P31025

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, saliva

Detection Range

1.56 ng/mL-100 ng/mL

Sensitivity

0.39 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LCN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Recovery

The recovery of human LCN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	92	90-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.714	2.768	2.741	2.654
50	1.812	1.801	1.807	1.720
25	1.005	1.058	1.032	0.945
12.5	0.538	0.568	0.553	0.466
6.25	0.301	0.325	0.313	0.226
3.12	0.245	0.238	0.242	0.155
1.56	0.142	0.156	0.149	0.062
0	0.088	0.086	0.087

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human LCN1 ELISA Kit was designed for the quantitative measurement of Human LCN1 protein in serum, plasma, tissue homogenates, saliva. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Target Background

Function
(From Uniprot)

Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket.

Gene References into Functions

At the relatively small concentrations in tears, all ceramides were complexed to tear lipocalin. PMID: 29331331
Human neutrophil lipocalin (HNL) concentrations in serum or whole blood activated by formyl-methionine-leucine-phenylalanine (fMLP) were shown to distinguish acute infections of bacterial or viral cause with high accuracy. PMID: 26135974
the possible relationship between ocular symptomatology, tear volume and tear break-up time (TBUT) and lipocalin, lactoferrin and lysozyme concentrations in the tear film were explored in a group of symptomatic dry-eyed postmenopausal (PM) women. PMID: 26366224
Tear lysozyme, lactoferrin and lipocalin concentrations were determined via electrophoresis and the results for patients with or without eyelid tumors were compared. PMID: 25914748
the crosstalk of dendritic cells with lipocalins alone has the potential to direct the type of immune response to these particular antigens. PMID: 26218644
Serum concentrations of LCN1 and LCN2 were both elevated in patients with COPD. PMID: 24920892
Tear lipocalin (TLC) is a major protein in tears and has a large ligand-binding cavity that allows the lipocalin to bind an extensive and diverse set of lipophilic molecules. PMID: 21791187
Study has identified the first protein, lipocalin-1, in the secretome of human blastocysts that is associated with chromosome aneuploidy. PMID: 21324447
The conserved disulfide bond of human tear lipocalin modulates conformation and lipid binding in a ligand selective manner.( PMID: 21466861
Tear lipocalin with the bound artificial ligand 1,4-butanediol has been crystallized in space group P2(1) with four protein molecules in the asymmetric unit and its X-ray structure has been solved at 2.6 A resolution. PMID: 19770509
The N-terminal part of recombinant human tear lipocalin/von Ebner's gland protein confers cysteine proteinase inhibition depending on the presence of the entire cystatin-like sequence motifs. PMID: 11727836
Lcn-1 is specifically produced by corticotrophs PMID: 11850445
LIMR mediating internalization of lipocalin-1 (Lcn-1) in NT2 cells, leading to its degradation. PMID: 12591932
structure, function, and mechanism of action [review] PMID: 12613960
Tear lipocalin is probable protein carrier for vitamin E tears and is candidate to specifically deliver vitamin E as antioxidant to corneal epithelium. PMID: 12613961
Tear surface tension depends partly on presence of tear lipocalin/lipid complex. Tear proteins and lipid-free tear lipocalin lower surface tension. PMID: 12613963
pH-driven ligand release involves ionization changes in several titratable residues associated with CD and EF loop apposition and occlusion of the calyx PMID: 15461462
structural analysis sheds new light on the ligand binding activity of this functionally obscure but abundant human lipocalin PMID: 15489503
Lipocalin is a novel autoantigen target in Sjogren's syndrome. PMID: 16249071
Hydrodynamic and solution properties of lipocalin from human tears show that the protein size and topology are the major determinants of rotational correlation time and a reason for deviation from the Stokes-Einstein relationship. PMID: 17869594
Selective displacement of ANS molecules from the ANS-apoTL complex by stearic acid discriminates the internal and external binding sites. PMID: 17945179
Tear lipocalin is the principal endonuclease in human tear fluid. PMID: 18334931
Results suggest that the excited protein states in tear lipocalin are not unique but consist of many substates. PMID: 19586017

Hide All

Subcellular Location

Secreted.

Protein Families

Calycin superfamily, Lipocalin family

Tissue Specificity

Mainly expressed in lachrymal and salivary glands. Also expressed in the prostate.

Database Links

HGNC: 6525

OMIM: 151675

KEGG: hsa:3933

STRING: 9606.ENSP00000263598

UniGene: Hs.530311

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-EL012810HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.