Human MHC class I polypeptide-related sequence B(MICB) ELISA kit

Instructions
Code CSB-EL013813HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name MHC class I polypeptide-related sequence B
Alternative Names MHC class I chain related protein B ELISA Kit; MHC class I chain related protein B2 ELISA Kit; MHC class I like molecule PERB11.2 IMX ELISA Kit; MHC class I mic B antigen ELISA Kit; MHC class I polypeptide related sequence B ELISA Kit; MHC class I polypeptide-related sequence B ELISA Kit; MIC B ELISA Kit; MIC-B ELISA Kit; MICB ELISA Kit; MICB_HUMAN ELISA Kit; PERB11.2 ELISA Kit; Stress inducible class I homolog ELISA Kit
Abbreviation MICB
Uniprot No. Q29980
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MICB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 82-95
1:2 Average % 99
Range % 93-104
1:4 Average % 95
Range % 89-99
1:8 Average % 94
Range % 88-98
Recovery
The recovery of human MICB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 94-101
EDTA plasma (n=4) 86 82-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.364 2.477 2.421 2.267
1000 1.767 1.654 1.711 1.557
500 1.081 1.136 1.109 0.955
250 0.713 0.724 0.719 0.565
125 0.442 0.455 0.449 0.295
62.5 0.371 0.367 0.369 0.215
31.25 0.252 0.259 0.256 0.102
0 0.153 0.154 0.154  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Gene References into Functions
  1. allele MICA*002:01/A9 and haplotype MICA*002:01-MICB*005:02 were negatively associated with respiratory syncytial virus respiratory tract infections. PMID: 28925058
  2. the present study revealed that DNA damagedependent MICA/B expression in insensitive cancer cells can be restored by chromatin relaxation via the HDAC/Suv39/G9a pathway. Collectively, manipulation of chromatin status by therapeutic cancer drugs may potentiate the antitumor effect by enhancing immune activation following radiotherapy and DNA damage-associated chemotherapy. PMID: 28677817
  3. The results of this study suggested that the MICB*009N allele might be a risk factor for SLE, whereas the MICB*014, MICA*010 and MICB*002 alleles were associated with reduced incidence of SLE in the study population. PMID: 29078849
  4. No association between MICB genotype and early viremia level in dengue patients. PMID: 28599625
  5. This demonstrates for the first time that MICA/B is more broadly expressed in normal tissue and that expression is mainly intracellular with only a small fraction appearing on the cell surface of some epithelia and tumour cells. PMID: 28334733
  6. this study shows that vigilin (HDLBP) binds and negatively regulates MICB expression through its 5'UTR, and that vigilin downregulation in target cells leads to NK cell activation against said target cells PMID: 28356383
  7. we have found that the allele G of rs3828903 was significantly associated with risk susceptibility to SLE in the current population. These data together with the functional annotations of rs3828903 convert MICB into a main candidate for being an additional MHC gene associated with SLE susceptibility. PMID: 27433477
  8. this study presents the impact of a single nucleotide transition at SNPs associated with DSS on differential protein binding patterns with PLCE1 and MICB genes and on protein structure modification and their possible role in the pathogenesis of dengue shock syndrome PMID: 27038471
  9. there is no obvious correlation between the MMP9 -1562 C/T SNP and the concentrations of circulating MICA/B in the breast cancer patients. PMID: 27026046
  10. Findings shown here are important from an anthropologic perspective and will inform future studies of the potential role of MICA and MICB genes in allogeneic organ transplantation and HLA-linked disease association in populations of related ancestry. PMID: 27028549
  11. IMP3 directly interacts with ULBP2 mRNA, leading to ULBP2 transcript destabilization and reduced ULBP2 surface expression and indirectly downregulates MICB with a mechanism functionally distinct from that of ULBP2. PMID: 26982091
  12. Reversal of epigenetic silencing of MICA and MICB improves immune recognition and killing of Merkel cell carcinoma cells. PMID: 26902929
  13. We have now found a total of 15 individual MICB promoter sequences, varying by combination of 18 polymorphic positions within the MICB minimal promoter sequence. PMID: 26707708
  14. Our results suggest that locally sustained expression of MICA and MICB in the tumor may contribute to the malignant progression of Gastric cancer(GC) and that expression of these ligands predicts an unfavorable prognosis in GC patients presenting large tumors. PMID: 26708143
  15. Estrogen upregulates MICA/B expression in human non-small cell lung cancer through the regulation of ADAM17. PMID: 25363527
  16. Aligned with MICB*005:02, MICB*030 has a nonsynonymous adenine substitution at nucleotide position 50 in exon 3, leading to amino acid change from serine to arginine at codon 102 of the mature MICB molecule. PMID: 25990310
  17. We have demonstrated a novel mechanism by which tumor-derived sMIC may temper the immune reactive tumor microenvironment. PMID: 25887583
  18. This study suggests that NKG2D ligands shedding of MICA, MICB and ULBP-2 is a novel pathway in endometriosis complex pathogenesis that impairs Natural Killer Cells cell function. PMID: 25775242
  19. six RNA-binding proteins that bind and regulate the expression of MICB. PMID: 24924487
  20. The sMICB serum levels were significantly increased in hepatitis B patients compared to healthy controls. The sMICB serum levels were decreased in a treated hepatocellular carcinoma (HCC) patient group compared to a not-treated HCC patient group. PMID: 25626490
  21. MICA/B expression is inhibited by unfolded protein response and associated with poor prognosis in human hepatocellular carcinoma. PMID: 25228093
  22. Genotoxic stress induces senescence-associated ADAM10-dependent release of NKG2D MIC ligands (MICA, MICB) in multiple myeloma cells. PMID: 26071561
  23. Data indicate that MHC class I chain-related protein A (MICA)( *)010, MICA( *)A5 and MHC class I chain-related protein B (MICB)( *)005:02 were the most frequent alleles. PMID: 25167773
  24. The elevation of soluble MICB during acute primary Dengue virus infections in infants likely represents an immune evasion strategy and contributes to the severity of the acute illness. PMID: 24869966
  25. If differential expression by polymorphic MICA and MICB promoters is confirmed by functional studies, involvement of these genes in disease susceptibility or adverse transplantation outcomes may require knowledge of both promoter and allelic types to make meaningful conclusions. PMID: 24962621
  26. MICb expression in macrophage foam cells infiltrating atherosclerotic plaques PMID: 24997223
  27. Experiments demonstrate that infection in vitro with human cytomegalovirus leads to enhanced shedding of MICA and MICB. PMID: 24973455
  28. Poorly differentiated tumors showed high MICA/B expression, which was related to extended tumor lymph node metastases and less frequent long-term survival. PMID: 24173243
  29. we conclude that rs3132468-C at MICB and rs3765524-C at PLCE1 confer risk of DSS in Southeast Asians. PMID: 24884822
  30. Broad MICA and MICB expression in the small bowel mucosa suggest a link between cellular stress and celiac disease. PMID: 24058482
  31. Hepatitis B surface antigen inhibits MICA and MICB expression via induction of cellular miRNAs in hepatocellular carcinoma cells. PMID: 23917076
  32. Diverse MICB alleles differentially bound cytomegalovirus UL16. PMID: 23625227
  33. the MICB rs3132468 and PLCE1 rs3740360 genotypes are associated with clinically apparent dengue in both adults and children. PMID: 23536857
  34. Soluble and membrane-restricted NKG2D ligand, MICB, poses opposite impacts on prostate cancer progression. PMID: 24018560
  35. study observed a substantial heterogeneity among the different cancer cell lines with regard to the involvement of ADAM10 and/or ADAM17 in MICA or MICB shedding PMID: 23526433
  36. 17beta-estradiol upon forming a complex with its cognate receptor suppresses MICB expression through binding with SP1/SP3 sites within the MICB promoter GC box. PMID: 23527639
  37. The frequencies of 20 MICB alleles and their haplotypes among 400 unrelated Han persons was determined. 5 new alleles, MICB*005:07, MICB*005:08, MICB*027, MICB*028, & MICB*029 were found.MICB*028 probably arose from MICB*004:01:01. PMID: 23549730
  38. MICA and MICB were upregulated in esophageal cancer. The expression of MICA/B was related with histological grade. PMID: 23291067
  39. Two new MICB alleles, MICB*005:06 and MICB*026, have been identified in a southern Chinese Han population. PMID: 22609444
  40. our results define MICB as a novel immune target of miR-10b PMID: 22915757
  41. Expression of FimX produces bidirectional inversion of the hyxR promoter region and promotes HyxR expression. HyxR suppresses intracellular survival in macrophages through reactive nitrogen intermediate-dependent and -independent mechanisms. PMID: 22221182
  42. MICA and MICB molecules act as key ligands for activating receptor natural killer cell (NK) group 2, member D (NKG2D) and promote NK cell-mediated recognition and cytolysis. PMID: 22301547
  43. high expression of MICB gene in Multiple sclerosis (MS) patients is an important criterion of MS disease that it may be due to the interaction between MICB and its receptor on CD8+T or NK cells. PMID: 22184268
  44. data suggest that posttranslational N-linked glycosylation is strictly required for NKG2D ligand, MICB, surface expression PMID: 22227571
  45. Data show that VSV infection caused an active suppression of NKG2D-ligand surface expression, affecting both endogenous and histone deacetylase (HDAC)-inhibitor induced MICA, MICB and ULBP-2 expression. PMID: 21857986
  46. The positive expression rate of MHC-I A/B and HLA-E in ALL patients was obviously higher than that in AML patients. PMID: 21518478
  47. Statistical analysis revealed a tendency for MICA*008 and MICB*008 to associate with susceptibility to illness when symptomatic versus asymptomatic cases of Dengue were compared. PMID: 21762746
  48. identified a susceptibility locus at MICB (major histocompatibility complex (MHC) class I polypeptide-related sequence B), which was within the broad MHC region on chromosome 6 but outside the class I and class II HLA loci, per-allele odds ratio PMID: 22001756
  49. Information about MICB allele frequencies and haplotypes with HLA in Koreans. PMID: 21554252
  50. The levels of MICB expression in serum and tissue of pancreatic cancer are elevated PMID: 21605422

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Involvement in disease Rheumatoid arthritis (RA)
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families MHC class I family, MIC subfamily
Tissue Specificity Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in s
Database Links

HGNC: 7091

OMIM: 180300

KEGG: hsa:4277

STRING: 9606.ENSP00000252229

UniGene: Hs.731446

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